Results 91 to 100 of about 43,663 (164)

CRISPR/Cas9-Assisted Transformation-Efficient Reaction (CRATER) for Near-Perfect Selective Transformation [PDF]

The CRISPR (Clustered, Regularly Interspaced, Short Palindromic Repeats)/Cas9 system has revolutionized genome editing by providing unprecedented DNA-targeting specificity.
Calvet, Charles, Devlin, Tyler D., Fujishima, Kosuke, Greenberg, Daniel T., Ju, Tina, Kent, Ryan E., Kunin, Daniel, Lieberman, Erica, Maheshwari, Akshay J., McCutcheon, Griffin, Nguyen, Thai, Rothschild, Lynn J., Shih, Joseph D., Takahashi, Jack R., Thompson, Kirsten A., Tran, Forrest, Xiang, Daniel   +16 more
core   +1 more source

Type II and type V CRISPR effector nucleases from a structural biologist’s perspective [PDF]

, 2016
The type II and type V CRISPR effector nucleases Cas9 and Cpf1 are “universal” DNA endonucleases, which can be programmed by an appropriate crRNA or sgRNA strand to cleave almost any DNA duplex at a preselected position (constrained only by short, so ...
Bochtler, Matthias, Fernandes, Humberto, Pastor, Michał   +2 more
core  

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Science, 2012
M. Jinek, Krzysztof Chylinski, Ines Fonfara, Michael H. Hauer, J. Doudna, E. Charpentier   +5 more
semanticscholar   +1 more source

CRISPR-Cas9 Structures and Mechanisms.

Annual Review of Biophysics, 2017
F. Jiang, J. Doudna
semanticscholar   +1 more source

A Simple Programmable Cas12a/crRNA Induced Walking System for Sensitive Methicillin-Resistant <i>Staphylococcus aureus</i> Detection via Integrated <i>cis</i>- and <i>trans</i>-Cleavage Activity. [PDF]

J Microbiol Biotechnol
Xiao B, Zhang J.
europepmc   +1 more source

A CRISPR Powered Immobilization-Free, Amplification-Free Carbon-Nanotube Field-Effect Transistor (FET) Sensor for Influenza A Virus (IAV). [PDF]

Molecules
Li W, Shi Y, Li D, Wang Y, Sun Y, Li H, Han Y.   +6 more
europepmc   +1 more source

A single-tube RAA-CRISPR-Cas12a diagnostic platform for rapid detection of Chlamydia trachomatis. [PDF]

J Biol Eng
Jiang T, He P, Guo Y, Liu H, Lu G, Wang Z.   +5 more
europepmc   +1 more source

Steric regulation of CRISPR/Cas12a trans-cleavage kinetics via split-activator extensions. [PDF]

Nucleic Acids Res
Zhang J, He X, Huang J, Cheng C, He G, Xia R, Yang J, Chen J, Guo L, Xiang D, Li F, Shi J, Li P.   +12 more
europepmc   +1 more source

CRISPR anti-tag-mediated room-temperature RNA detection using CRISPR/Cas13a. [PDF]

Nat Commun
Moon J, Zhang J, Guan X, Yang R, Guo C, Schalper KT, Avery L, Banach D, LaSala R, Warrier R, Liu C.   +10 more
europepmc   +1 more source

Simple and Sensitive <i>Escherichia coli</i> Analysis <i>via</i> Allosteric Probe Controllable Switch Cas12a/crRNA Complex Mediated Strategy. [PDF]

J Microbiol Biotechnol
Jiang Y, Zhao C, Fang X, Shi X, Qi H.
europepmc   +1 more source