Results 141 to 150 of about 43,663 (164)

CRISPR/Cas12a DTR system: a topology-guided Cas12a assay for specific dual detection of RNA and DNA targets. [PDF]

Nucleic Acids Res
Jiang Q, Jin S, Qin Z, Zhang J, He R, Chen Z, Qiao B, Qiao J, Liu Y.   +8 more
europepmc   +1 more source

Programmable molecular microscopy: CRISPR/Cas fluorescent probes revolutionizing spatiotemporal genomic imaging. [PDF]

Theranostics
Zhong XY, Yang YX, Xiong YF, Ye GC, Gong X, Zhong ML, He HD, Wang SG, Xia QD.   +8 more
europepmc   +1 more source

RNA structure modulates Cas13 activity and enables mismatch detection. [PDF]

Nat Biotechnol
Larsen BB, Kimchi O, Dunkley ORS, Grimm MS, Siegers JY, Huang Y, Vandavasi VG, Nguyen LT, Lamb CH, Aranovich I, Eggink D, Meijer A, Jalal H, Notterman DA, Karlsson EA, Te Velthuis AJW, Myhrvold C.   +16 more
europepmc   +1 more source

Detection of rifampin-resistant Mycobacterium tuberculosis using CRISPR/Cas14a-enabled molecular techniques. [PDF]

BMC Infect Dis
Wu G, Ren Y, Wang Y, Zhao Y, Wu Y, Sun H, Sun Z, Wang R, Du Z.   +8 more
europepmc   +1 more source

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Foldback-crRNA-Enhanced CRISPR/Cas13a System (FCECas13a) Enables Direct Detection of Ultrashort sncRNA.

Analytical Chemistry, 2023
The CRISPR/Cas13a system has promising applications in clinical small noncoding RNA (sncRNA) detection because it is free from the interference of genomic DNA.
Yong Chen, Yibin Zhang, Siyuan Luo, Xinyao Yang, Conghui Liu, Qianling Zhang, Yizhen Liu, Xueji Zhang   +7 more
semanticscholar   +1 more source

Fluorescence and Colorimetric Analysis of African Swine Fever Virus Based on the RPA-Assisted CRISPR/Cas12a Strategy.

Analytical Chemistry, 2023
It is well-established that different detection modes are necessary for corresponding applications, which can effectively reduce matrix interference and improve the detection accuracy.
Guobin Mao, Xing Luo, Silu Ye, Xun Wang, Jin He, J. Kong, Junbiao Dai, Wen Yin, Yingxin Ma   +8 more
semanticscholar   +1 more source

Programmable Aptasensor for Regulating CRISPR/Cas12a Activity.

ACS Sensors, 2023
CRISPR-mediated aptasensors have gained prevalence for detecting non-nucleic acid targets. However, there is an urgent need to develop an easily customizable design to improve the signal-to-noise ratio, enhance universality, and expand the detection ...
Yao Yu, Qiaoyu Li, Wen Shi, Yuxin Yang, Hongpeng He, Junbiao Dai, Guobin Mao, Yingxin Ma   +7 more
semanticscholar   +1 more source

Development of an inducible Cas9 nickase and PAM-free Cas12a platform for bacterial diagnostics.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2023
Rapid, efficient, specific and sensitive diagnostic techniques are critical for selecting appropriate treatments for drug-resistant bacterial infections.
Yuanzhao Hu, Yuefeng Qiao, Xiu-Qing Li, Zhenbo Xiang, Yi Wan, Peng Wang, Zhiqing Yang   +6 more
semanticscholar   +1 more source