Results 151 to 160 of about 43,663 (164)

Dual-Accelerated Signal Amplification in Biosensing via Spatial Confining Catalytic Hairpin Assembly-Activated Spherical CRISPR/Cas12a System for Trans-Cleavage of Hairpin DNA Reporters.

Analytical Chemistry
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression and are implicated in various diseases, including cancer. Due to their critical role in diagnostics, there is a growing need for sensitive, specific, and rapid detection methods ...
Zhuqi Sui, B. Chen, Jia Zhao, Haidong Yang, Longhua Guo, Jianguo Xu   +5 more
semanticscholar   +1 more source

Abstract PD1-03: Estrogen receptor is a target of enzalutamide in ER+ breast cancer

Cancer Research, 2022
Background: Androgen receptor (AR) is widely expressed in ER+ breast cancers. Accumulated evidence showed that AR is a tumor suppressor in ERα+ breast cancers.
Lixuan Wei, Jia Yu, Huanyao Gao, Huan Zhang, T. Nguyen, Yayun Gu, R. Weinshilboum, J. Ingle, Liewei Wang   +8 more
semanticscholar   +1 more source

CRISPR/Cas12a-Powered Electrochemical Platform for Dual-miRNA Detection via an AND Logic Circuit.

Analytical Chemistry
The CRISPR/Cas technology shows great potential in molecular detection and diagnostics. However, it is still challenging to detect multiple targets simultaneously using the CRISPR-Cas system.
Jiangbo Dong, Changjun Hou, Liyuan Deng, Tao Gu, Shuyu Zhu, Jingzhou Hou, D. Huo   +6 more
semanticscholar   +1 more source

Evaluation of a D-octaarginine-linked polymer for CRISPR-Cas9 ribonucleoprotein (RNP) delivery and genome editing in murine dendritic cells.

Journal of Veterinary Medical Science
We previously reported that poly (N-vinylacetamide-co-acrylic acid) coupled with d-octaarginine (VP-R8) efficiently introduces proteins and nucleic acids into cells.
Takayuki Shimizu, Mariko Okamoto, Keiko Kawamoto   +2 more
semanticscholar   +1 more source

Application of CRISPR–Cas9 technology in the treatment of chronic lymphocytic leukemia with TP53 mutations

Cancer Plus
This study proposes the implementation of clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) technology for gene therapy targeting genetic mutations in human lymphocytes affected by chronic lymphocytic ...
A. Udristioiu, Liviu Martin, Delia Nica-Badea, A. V. Tetileanu, Manole Cojocaru, I. Gheorghe   +5 more
semanticscholar   +1 more source

Synergistic Incorporation of Two ssDNA Activators Enhances the Trans-Cleavage of CRISPR/Cas12a.

Analytical Chemistry, 2023
Qian Li, Zhiling Song, Yuxi Zhang, Lina Zhu, Qian-Fan Yang, Xingfu Liu, Xufeng Sun, Xuxu Chen, Rongmei Kong, Gaochao Fan, Xiliang Luo   +10 more
semanticscholar   +1 more source

The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR–Cas subtype III-Bv system

Nature Microbiology, 2018
Juliane Behler, Kundan Sharma, Viktoria Reimann, A. Wilde, H. Urlaub, W. Hess   +5 more
semanticscholar   +2 more sources

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

Nature, 2016
Ines Fonfara, Hagen Richter, Majda Bratovič, Anaïs Le Rhun, E. Charpentier   +4 more
semanticscholar   +1 more source

CRISPR-Cas9-Mediated Genome Modifications in Zebrafish.

Methods in molecular biology, 2023
Y. Kamachi, A. Kawahara
semanticscholar   +1 more source

Fig. 4. CRISPR system: tracrRNA – trans-activating (guide) RNA; crRNA – trans-acting RNA that binds to DNA

semanticscholar   +1 more source