Results 11 to 20 of about 38,906 (120)
Cancer Communications, Volume 42, Issue 12, Page 1257-1287, December 2022., 2022 Abstract Clustered regularly interspaced short palindromic repeats‐associated protein (CRISPR/Cas9), an adaptive microbial immune system, has been exploited as a robust, accurate, efficient and programmable method for genome targeting and editing. This innovative and revolutionary technique can play a significant role in animal modeling, in vivo genome
Khaled S. Allemailem +6 more
wiley +1 more source
Nucleic acid nanostructure for delivery of CRISPR/Cas9‐based gene editing system
Interdisciplinary Medicine, Volume 1, Issue 1, January 2023., 2023 Multifunctional nucleic acid nanostructures, including RCA‐derived DNA, branched DNA, and hybrid DNA, have been developed for delivery of CRISPR/Cas9‐based gene editing system for gene therapy. Abstract CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein 9)‐based gene editing system has aroused great ...
Wantao Tang, Jianbing Liu, Baoquan Ding
wiley +1 more source
CRISPR applications for Duchenne muscular dystrophy: From animal models to potential therapies
WIREs Mechanisms of Disease, Volume 15, Issue 1, January/February 2023., 2023 CRISPR‐Cas9 gene‐editing technology enables the rapid generation of animal models for Duchenne muscular dystrophy research and has potential to be developed as CRISPR therapy for the long lasting genetic correction of causal mutations. Abstract CRISPR gene‐editing technology creates precise and permanent modifications to DNA.
Yu C. J. Chey +4 more
wiley +1 more source
Reprograming of proteasomal degradation by branched chain amino acid metabolism
Aging Cell, Volume 21, Issue 12, December 2022., 2022 Using genetics and multi‐omics analyses in Caenorhabditis elegans, we identified adaptive regulation of the ubiquitin‐proteasome system (UPS) in response to defective branched chain amino acid (BCAA) metabolism. Worms with impaired BCAA metabolism show slower turnover of a GFP‐based proteasome substrate, which is suppressed by loss‐of‐function of the ...
Sonia Ravanelli +5 more
wiley +1 more source
Andrology, EarlyView., 2023 Abstract Background Mammalian fertilization is mediated by multiple sperm acrosomal proteins, many of which are testis‐enriched transmembrane glycoproteins expressed during spermiogenesis (e.g., Izumo sperm‐egg fusion 1, Sperm acrosome associated 6, and Transmembrane protein 95).
Yo Ogawa +4 more
wiley +1 more source
Crispr Cas System in Plant Genome Editing a New Opportunity in Agriculture to Boost Crop Yield
UMYU Journal of Microbiology Research, 2018 Clustered regularly interspaced short palindromic repeats CRISPR/Cas9 technology evolved from a type II bacterial immune system develop in 2013 This system employs RNA-guided nuclease, CRISPR associated (Cas9) to induce double-strand breaks.
Sunusi, M. +3 more
doaj +1 more source
Experimental animals, 2016 The CRISPR/Cas9 system is a powerful genome editing tool for the production of genetically modified animals. To produce mutant mice, chimeric single-guide RNA (sgRNA) is cloned in a plasmid vector and a mixture of sgRNA and Cas9 are microinjected into ...
Miho Terao +5 more
semanticscholar +1 more source
Distinct horizontal transfer mechanisms for type I and type V CRISPR-associated transposons
bioRxiv, 2023 CRISPR-associated transposons (CASTs) co-opt CRISPR-Cas proteins and Tn7-family transposons for RNA-guided vertical and horizontal transmission. CASTs encode minimal CRISPR arrays but can’t acquire new spacers.
semanticscholar +1 more source
crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus
RNA Biology, 2013 The Cas9-crRNA complex of the Streptococcus thermophilus DGCC7710 CRISPR3-Cas system functions as an RNA-guided endonuclease with crRNA-directed target sequence recognition and protein-mediated DNA cleavage.
Tautvydas Karvelis +5 more
semanticscholar +1 more source
The tracrRNA in CRISPR Biology and Technologies.
Annual Review of Genetics, 2021 CRISPR-Cas adaptive immune systems in bacteria and archaea utilize short CRISPR RNAs (crRNAs) to guide sequence-specific recognition and clearance of foreign genetic material.
Chunyu Liao, Chase L. Beisel
semanticscholar +1 more source