CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
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Nature Biotechnology, 2021 Gene therapy would benefit from a miniature CRISPR system that fits into the small adeno-associated virus (AAV) genome and has high cleavage activity and specificity in eukaryotic cells.
Do Yon Kim +9 more
semanticscholar +1 more source
A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
, 2015 Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
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Comparative genomics and evolution of trans-activating RNAs in Class 2 CRISPR-Cas systems
RNA Biology, 2018 Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems.
Guilhem Faure +6 more
semanticscholar +1 more source
CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method. [PDF]
, 2017 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed.
Chen, Chao +6 more
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Structural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9
Science, 2022 Class 2 CRISPR effectors Cas9 and Cas12 may have evolved from nucleases in IS200/IS605 transposons. IscB is about two-fifths the size of Cas9 but shares a similar domain organization.
G. Schuler, Chunyi Hu, A. Ke
semanticscholar +1 more source
Experimental animals, 2016 The CRISPR/Cas9 system is a powerful genome editing tool for the production of genetically modified animals. To produce mutant mice, chimeric single-guide RNA (sgRNA) is cloned in a plasmid vector and a mixture of sgRNA and Cas9 are microinjected into ...
Miho Terao +5 more
semanticscholar +1 more source
Action mechanisms of CRISPR/Cas system and its application in genetic improvement of crops
浙江大学学报. 农业与生命科学版, 2018 CRISPR/Cas system is an emerging gene-editing technology, which can knock out multiple genes at multiple specific loci. Compared with other gene editing systems including ZFNs (zinc finger nucleases) and TALENs (transcription activator-like effector ...
SHU Xinyuan +4 more
doaj +1 more source
Molecular mechanisms of CRISPR-mediated microbial immunity [PDF]
, 2013 Bacteriophages (phages) infect bacteria in order to replicate and burst out of the host, killing the cell, when reproduction is completed. Thus, from a bacterial perspective, phages pose a persistent lethal threat to bacterial populations.
Giedrius Gasiunas +2 more
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dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression. [PDF]
, 2017 Distinct epigenomic profiles of histone marks have been associated with gene expression, but questions regarding the causal relationship remain. Here we investigated the activity of a broad collection of genomically targeted epigenetic regulators that ...
Farnham, Peggy J +8 more
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