Results 21 to 30 of about 43,663 (164)

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

RNA Biology, 2013
The Cas9-crRNA complex of the Streptococcus thermophilus DGCC7710 CRISPR3-Cas system functions as an RNA-guided endonuclease with crRNA-directed target sequence recognition and protein-mediated DNA cleavage.
Tautvydas Karvelis, G. Gasiunas, Algirdas Miksys, R. Barrangou, P. Horvath, V. Šikšnys   +5 more
semanticscholar   +1 more source

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ [PDF]

, 2016
Background: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in.
Aida, Tomomi, Aizawa, Hidenori, Ishikubo, Harumi, Izu, Yayoi, Mochida, Keiji, Nakade, Shota, Oishi, Ayu, Sakuma, Tetsushi, Tanaka, Kohichi, Usami, Takako, Yamamoto, Takashi   +10 more
core   +1 more source

The tracrRNA in CRISPR Biology and Technologies.

Annual Review of Genetics, 2021
CRISPR-Cas adaptive immune systems in bacteria and archaea utilize short CRISPR RNAs (crRNAs) to guide sequence-specific recognition and clearance of foreign genetic material.
Chunyu Liao, Chase L. Beisel
semanticscholar   +1 more source

Genome editing technologies to fight infectious diseases [PDF]

, 2017
Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa, Pal\ue3\ub9, Giorgio, Trevisan, Marta   +2 more
core   +1 more source

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system [PDF]

, 2013
The ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties.
Bikard, David, Hochschild, Ann, Jiang, Wenyan, Marraffini, Luciano A., Samai, Poulami, Zhang, Feng   +5 more
core   +2 more sources

Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain

bioRxiv, 2022
Nucleases derived from the prokaryotic defense system CRISPR-Cas are frequently re-purposed for gene editing and molecular diagnostics. Hence, an in-depth understanding of the molecular mechanisms of these enzymes is of crucial importance.
Elisabeth Wörle, A. Newman, G. Burgio, Dina Grohmann   +3 more
semanticscholar   +1 more source

Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing [PDF]

, 2014
The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but can mediate only a single activity at a time within any given cell.
Braff, Jonathan L., Church, George M., Esvelt, Kevin M., Mali, Prashant, Moosburner, Mark, Yaung, Stephanie J.   +5 more
core   +1 more source

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes

Genetics, 2015
Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs.
Alexandre Paix, Andrew W. Folkmann, D. Rasoloson, G. Seydoux   +3 more
semanticscholar   +1 more source

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3 [PDF]

, 2014
Mobile genetic elements in bacteria are neutralized by a system based on clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins.
Bolt, Edward L., Gong, Bei, Jung, Che-Hun, Kim, Jeong-Sun, Shin, Minsang, Sun, Jiali, van der oost, John   +6 more
core   +3 more sources

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems [PDF]

, 2017
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and Cpf1, are known.
Abudayyeh, Omar Osama, Gootenberg, Jonathan S, Joung, Julia, Konermann, Silvana M, Koonin, Eugene V., Makarova, Kira S., Minakhin, Leonid, Semenova, Ekaterina, Severinov, Konstantin, Shmakov, Sergey, Wolf, Yuri I., Zhang, Feng   +11 more
core   +1 more source