Results 21 to 30 of about 38,906 (120)
bioRxiv, 2022 Nucleases derived from the prokaryotic defense system CRISPR-Cas are frequently re-purposed for gene editing and molecular diagnostics. Hence, an in-depth understanding of the molecular mechanisms of these enzymes is of crucial importance.
Elisabeth Wörle +3 more
semanticscholar +1 more source
A small PAM optimises target recognition in the CRISPR-Cas immune system [PDF]
arXiv, 2021 CRISPR-Cas is an adaptive immune mechanism that has been harnessed for a variety of genetic engineering applications: the Cas9 protein recognises a 2-5nt DNA motif, known as the PAM, and a programmable crRNA binds a target DNA sequence that is then cleaved.
arxiv
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Science, 2012 Ditching Invading DNA Bacteria and archaea protect themselves from invasive foreign nucleic acids through an RNA-mediated adaptive immune system called CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas).
M. Jinek +5 more
semanticscholar +1 more source
CRISPRtracrRNA: robust approach for CRISPR tracrRNA detection
Bioinform., 2022 Motivation The CRISPR-Cas9 system is a Type II CRISPR system that has rapidly become the most versatile and widespread tool for genome engineering. It consists of two components, the Cas9 effector protein, and a single guide RNA that combines the spacer (
A. Mitrofanov +4 more
semanticscholar +1 more source
Rapid and Accurate Detection of SARS-CoV-2 Mutations using a Cas12a-based Sensing Platform [PDF]
arXiv, 2021 The increasing prevalence of SARS-CoV-2 variants with spike mutations has raised concerns owing to higher transmission rates, disease severity, and escape from neutralizing antibodies. Rapid and accurate detection of SARS-CoV-2 variants provides crucial information concerning the outbreaks of SARS-CoV-2 variants and possible lines of transmission. This
arxiv
CRISPR-Cas9 Structures and Mechanisms.
Annual Review of Biophysics, 2017 Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems employ the dual RNA-guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific ...
F. Jiang, J. Doudna
semanticscholar +1 more source
Template-based eukaryotic genome editing directed by SviCas3 [PDF]
arXiv, 2023 RNA-guided gene editing based on the CRISPR-Cas system is currently the most effective genome editing technique. Here, we report that the SviCas3 from the subtype I-B-Svi Cas system in Streptomyces virginiae IBL14 is an RNA-guided and DNA-guided DNA endonuclease suitable for the HDR-directed gene and/or base editing of eukaryotic cell genomes.
arxiv
Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons
bioRxiv, 2022 CRISPR systems have been co-opted by Tn7-like elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the AAA+ ATPase TnsC, the Zn-finger protein TniQ, and the ...
M. Schmitz +4 more
semanticscholar +1 more source
Genetics, 2015 Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs.
Alexandre Paix +3 more
semanticscholar +1 more source
Cell Reports, 2020 Summary: In CRISPR-Cas9-assisted knockin (KI) in zygotes, a remaining challenge is routinely achieving high-efficiency KI of large (kilobase-sized) DNA elements.
Takaya Abe +3 more
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