Results 31 to 40 of about 38,906 (120)
Early “reduction to practice” of the CRISPR–Cas9 invention in eukaryotic cells
Frontiers in Genetics, 2022 The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)–Cas (CRISPR-associated proteins) are microbial adaptive immune mechanisms that have revolutionized many areas of Life Sciences research and innovation and potentially could transform ...
G. Aquino-Jarquin
semanticscholar +1 more source
Advanced Science, EarlyView.This work designs manganese metal‐organic frameworks (Mn‐MOFs) co‐assembled with Cas12a/crRNA, enabling rapid release of Mn2⁺ under mild conditions. The MOFs‐induced proximity effect sustains Mn2⁺‐Cas12a/crRNA interactions, enhancing the trans‐cleavage activity of complex available to develop an electrochemical biosensor based on Mn‐MOFs‐enhanced ...
Shuai Wu +8 more
wiley +1 more source
Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq.
Methods in molecular biology, 2021 CRISPR-Cas systems consist of a complex ribonucleoprotein (RNP) machinery encoded in prokaryotic genomes to confer adaptive immunity against foreign mobile genetic elements.
Sahil Sharma, C. Sharma
semanticscholar +1 more source
The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems
RNA Biology, 2013 CRISPR-Cas is a rapidly evolving RNA-mediated adaptive immune system that protects bacteria and archaea against mobile genetic elements. The system relies on the activity of short mature CRISPR RNAs (crRNAs) that guide Cas protein(s) to silence invading ...
Krzysztof Chylinski +2 more
semanticscholar +1 more source
Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing
Small, EarlyView.The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa +4 more
wiley +1 more source
Rapid Amplification and Detection of Single‐Stranded Nucleic Acids for Point‐of‐Care Diagnosis
Small Methods, EarlyView.A rapid, point‐of‐care nucleic acid test can greatly benefit healthcare system by providing timely diagnosis for effective treatment and patient management, as well as supporting diseases surveillance for emerging pandemic diseases. Recent advancements in nucleic acids technology have led to rapid assays for single‐stranded nucleic acids that can be ...
Jinglin Fu +6 more
wiley +1 more source
Small Structures, EarlyView.This illustration depicts a comprehensive strategy against monkeypox, showcasing its transmission, symptoms, and prevention. Centralized around nanomaterials and biosensors, it integrates early screening, Internet of Things, vaccination, and community involvement through collaboration among healthcare workers, scientists, researchers, social groups ...
Vishal Chaudhary +3 more
wiley +1 more source
Plant, Cell &Environment, EarlyView.ABSTRACT Pulses provide myriad health benefits and are advantageous in an environmental context as a result of their leguminous nature. However, phytopathogenic fungi, oomycetes and bacteria pose a substantial threat to pulse production, at times leading to crop failure.
Stacy D. Singer +6 more
wiley +1 more source
Application of Genome Editing Technologies for Disease Treatment: Review
, 2021 The improvement of particularly versatile genome-modifying advancements has outfitted experts with the ability to rapidly and monetarily bring sequence-specific changes into the genomes of a wide scope of cell types and organisms.
G. Belachew +2 more
semanticscholar +1 more source
Harnessing promoter elements to enhance gene editing in plants: perspectives and advances
Plant Biotechnology Journal, Volume 23, Issue 5, Page 1375-1395, May 2025.Summary Genome‐edited plants, endowed with climate‐smart traits, have been promoted as tools for strengthening resilience against climate change. Successful plant gene editing (GE) requires precise regulation of the GE machinery, a process controlled by the promoters, which drives its transcription through interactions with transcription factors (TFs ...
Nikita Gondalia +8 more
wiley +1 more source