Results 41 to 50 of about 43,663 (164)
Occurrence and diversity of CRISPR-Cas systems in the genus bifidobacterium [PDF]
, 2017 CRISPR-Cas systems constitute adaptive immune systems for antiviral defense in bacteria. We investigated the occurrence and diversity of CRISPR-Cas systems in 48 Bifidobacterium genomes to gain insights into the diversity and co-evolution of CRISPR-Cas ...
Barrangou, Rodolphe +3 more
core +1 more source
Droplet Digital CRISPR for Nucleic Acid Detection
Advanced Science, EarlyView.This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang +4 more
wiley +1 more source
The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems
RNA Biology, 2013 CRISPR-Cas is a rapidly evolving RNA-mediated adaptive immune system that protects bacteria and archaea against mobile genetic elements. The system relies on the activity of short mature CRISPR RNAs (crRNAs) that guide Cas protein(s) to silence invading ...
semanticscholar +1 more source
Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons
bioRxiv, 2022 CRISPR systems have been co-opted by Tn7-like elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the AAA+ ATPase TnsC, the Zn-finger protein TniQ, and the ...
M. Schmitz +4 more
semanticscholar +1 more source
MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection
Advanced Science, EarlyView.MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani +6 more
wiley +1 more source
Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C. [PDF]
, 2019 The unidirectional and opposite-polarity microtubule-based motors, dynein and kinesin, drive long-distance intracellular cargo transport. Cellular observations suggest that opposite-polarity motors may be coupled.
Dickey, Andrea M +7 more
core
Functional rescue of dystrophin deficiency in mice caused by frameshift mutations using Campylobacter jejuni Cas9 [PDF]
, 2018 Duchenne muscular dystrophy (DMD) is a fatal, X-linked muscle wasting disease caused by mutations in the DMD gene. In 51% of DMD cases, a reading frame is disrupted because of deletion of several exons.
Cappellari, O +9 more
core +2 more sources
Advanced Science, EarlyView.Biomimetic membrane interface engineering constructs functionalized detection platforms by integrating natural cell membranes, synthetic lipids, or hybrid membranes. This strategy effectively reduces background interference and enables efficient target capture and analysis, showing broad applications in circulating tumor cell separation, extracellular ...
Duo Liu +8 more
wiley +1 more source
Early “reduction to practice” of the CRISPR–Cas9 invention in eukaryotic cells
Frontiers in Genetics, 2022 The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)–Cas (CRISPR-associated proteins) are microbial adaptive immune mechanisms that have revolutionized many areas of Life Sciences research and innovation and potentially could transform ...
G. Aquino-Jarquin
semanticscholar +1 more source
Guidelines for Light‐Start One‐Pot RPA‐CRISPR‐Cas12a Assays for Foodborne Pathogen Detection
Food Safety and Health, EarlyView.Light‐start one‐pot RPA‐CRISPR‐Cas12a detection schematic diagram. (A) Linear crRNA activation light‐start one‐pot workflow. (B) Caged crRNA activation light‐start one‐pot workflow. ABSTRACT Rapid and accurate detection of nucleic acids of foodborne pathogens is of vital importance for controlling the spread of foodborne diseases and safeguarding ...
Fan Li +4 more
wiley +1 more source