Results 41 to 50 of about 38,906 (120)

Novel in vivo models of autosomal optic atrophy reveal conserved pathological changes in neuronal mitochondrial structure and function

The FASEB Journal, Volume 39, Issue 7, 15 April 2025.
Autosomal optic atrophy (AOA) is a hereditary condition caused by mutations in OPA1, leading to retinal ganglion cell (RGC) degeneration. This study introduces two novel models to study OPA1 loss in neurons, revealing reduced life span and impaired visual function.
Elin L. Strachan, Eugene T. Dillon, Mairéad Sullivan, Jeffrey C. Glennon, Amandine Peyrel, Jérôme Sarniguet, Kevin Dubois, Benjamin Delprat, Breandán N. Kennedy, Niamh C. O'Sullivan   +9 more
wiley   +1 more source

State‐of‐the‐art in food safety: The potential of CRISPR/Cas‐based lateral flow assay technology in the field of food analysis

Food Safety and Health, Volume 3, Issue 2, Page 155-171, April 2025.
The CRISPR‐LFA merges the CRISPR/Cas system with a LFA strip. This approach utilizes the programmability and specificity of the CRISPR/Cas system for detecting various nucleic acid molecules. Abstract Food safety is essential for public health, with key hazards including microbial and chemical contamination and pesticide residues.
Yang Sun, Kunli Zhong, Lanmengya Nie, Yan Xu, Yiran Wang, Jingtao Yuan, Shuai Jiang, Xu Li, Zhidong Zhang, Xiangfei Li, Ling Jiang   +10 more
wiley   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

A functional type II-A CRISPR–Cas system from Listeria enables efficient genome editing of large non-integrating bacteriophage

Nucleic Acids Research, 2018
CRISPR–Cas systems provide bacteria with adaptive immunity against invading DNA elements including bacteriophages and plasmids. While CRISPR technology has revolutionized eukaryotic genome engineering, its application to prokaryotes and their viruses ...
Mario Hupfeld, Despoina Trasanidou, Livia Ramazzini, J. Klumpp, M. Loessner, S. Kilcher   +5 more
semanticscholar   +1 more source

Recent Technologies for the Determination of SARS‐CoV‐2 in Wastewater

ChemistrySelect, Volume 10, Issue 13, April 4, 2025.
The integration of WBE with conventional techniques such as PCR, ELISA, LAMP and CRISPR; and electrochemical biosensors based on a variety of bioreceptors can be used as a large‐scale tool to monitor the spread of COVID‐19, especially in communities with limited resources worldwide.
Nolwazi T. Gazu, Aoife Morrin, Xolile Fuku, Bhekie B. Mamba, Usisipho Feleni   +4 more
wiley   +1 more source

DNA-guided CRISPR/Cas12 for RNA targeting

medRxiv
CRISPR-Cas nucleases are transforming genome editing, RNA editing, and diagnostics but have been limited to RNA-guided systems. We present {psi}DNA, a DNA-based guide for Cas12 enzymes, engineered for specific and efficient RNA targeting. {psi}DNA mimics
Carlos Orosco, S. Rananaware, Boyu Huang, Michael P Hanna, M. Ahmadimashhadi, Jordan G Lewis, Michael P Baugh, August P Bodin, Sarah J Flannery, Ian H Lange, Zoe R Fang, Vedant N Karalkar, Katelyn S. Meister, P. Jain   +13 more
semanticscholar   +1 more source

An Agrobacterium‐delivered CRISPR/Cas9 system for targeted mutagenesis in sorghum

Plant Biotechnology Journal, 2019
Clustered regularly interspaced short palindromic repeat/CRISPR-associated Cas9 (CRISPR/Cas9) systems of bacteria and archaea have engineered for genome editing in eukaryotic genomes.
Si Nian Char, Jialu Wei, Qi Mu, Xianran Li, Zhanyuan J. Zhang, Jianming Yu, Bing Yang   +6 more
semanticscholar   +1 more source

Establishment and characterization of adap1‐deficient zebrafish

Development, Growth &Differentiation, Volume 67, Issue 3, Page 165-173, April 2025.
To examine the physiological functions of the adap1 gene, we have established zebrafish adap1 mutants lacking functional PH1 and PH2 domains. The adap1 mutants showed no apparent morphological abnormalities and grew to adulthood. We found that the adult adap1 mutants spent more time than the wild type in the regions near the chambers where novel ...
Atsuo Kawahara, Sakyo Yasojima, Junko Koiwa, Saori Fujimaki, Hiroaki Ito, Mamiko Yamada, Kenjiro Kosaki, Yuhei Nishimura   +7 more
wiley   +1 more source

Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endonuclease*

Journal of Biological Chemistry, 2014
Background: The Cas9 RNA-guided endonuclease has been adapted for genome manipulation and regulation. Results: We have characterized target recognition and cleavage by Streptococcus thermophilus LMG18311 Cas9. Conclusion: The two nuclease domains of Cas9
Hongfan Chen, Ji-Sun Choi, S. Bailey
semanticscholar   +1 more source

Multi‐omics analysis identified the GmUGT88A1 gene, which coordinately regulates soybean resistance to cyst nematode and isoflavone content

Plant Biotechnology Journal, Volume 23, Issue 4, Page 1291-1307, April 2025.
Summary Soybean cyst nematode (SCN, Heterodera glycines) is a major pathogen harmful to soybean all over the world, causing huge yield loss every year. Soybean resistance to SCN is a complex quantitative trait controlled by a small number of major genes (rhg1 and Rhg4) and multiple micro‐effect genes.
Haipeng Jiang, Shuo Qu, Fang Liu, Haowen Sun, Haiyan Li, Weili Teng, Yuhang Zhan, Yongguang Li, Yingpeng Han, Xue Zhao   +9 more
wiley   +1 more source