Results 51 to 60 of about 43,663 (164)

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. [PDF]

, 2018
CRISPR-Cas systems provide microbes with adaptive immunity to infectious nucleic acids and are widely employed as genome editing tools. These tools use RNA-guided Cas proteins whose large size (950 to 1400 amino acids) has been considered essential to ...
Banfield, JF, Burstein, D, Chen, JS, Cofsky, JC, Doudna, JA, Harrington, LB, Kyrpides, NC, Ma, E, Paez-Espino, D, Witte, IP   +9 more
core  

Production and analysis of synthetic Cascade variants [PDF]

, 2020
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR assoziiert) ist ein adaptives Immunsystem in Archaeen und Bakterien, das fremdes genetisches Material mit Hilfe von Ribonukleoprotein-Komplexen erkennt und zerstört.
Gleditzsch, Daniel
core   +1 more source

Metagenomic mining reveals novel Cas12 subtypes and their evolutionary diversification

iMetaOmics, EarlyView.
We identified 4112 Cas12 Proteins and 6 new Cas12 subtypes, revealed their significant diversity in N‐terminal regions, repeat sequences, and sequences motifs. We developed an AI‐driven algorithm, Cas12fam, which allows precise annotation of 18 distinct domains in Cas12 proteins.
Meixia Yu, Zilong Zhao, Peiyang Li, Yunpeng Teng, Feng Xu, Zhimeng Li, Zhijia Yang, Hainan Zhao, Huanbin Zhou, Lanqin Xia, Jinsheng Lai, Beibei Xin   +11 more
wiley   +1 more source

WU-CRISPR: Characteristics of functional guide RNAs for the CRISPR/Cas9 system [PDF]

, 2015
The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome ...
Liu, Weijun, Wang, Xiaowei, Wong, Nathan
core   +2 more sources

CRISPR Enabled Precision Oncology: From Gene Editing to Tumor Microenvironment Remodeling

Med Research, EarlyView.
CRISPR technology has progressed from a prokaryotic immune system to a diverse suite of editing platforms, including Cas nucleases, base and prime editors, and RNA‐targeting enzymes. These advances enable precise genomic and epigenomic interventions, high‐throughput functional screening, and immune engineering.
Kailai Li, Peixin Huang, Yue Qian, Anqi Lin, Jingjun He, Junyi Shen, Li Chen, Kai Miao, Jian Zhang   +8 more
wiley   +1 more source

Studies of Single-Molecule Dynamics in Microorganisms [PDF]

, 2019
Fluorescence microscopy is one of the most extensively used techniques in the life sciences. Considering the non-invasive sample preparation, enabling live-cell compliant imaging, and the specific fluorescence labeling, allowing for a specific visualization
Turkowyd, Bartosz Daniel
core   +1 more source

Environmental DNA‐based RPA‐CRISPR/Cas12a assay for on‐site detection of chironomid larvae in aquatic environments

Pest Management Science, EarlyView.
An environmental DNA (eDNA)‐based recombinase polymerase amplification (RPA)‐CRISPR/Cas12a assay enables rapid, specific, and on‐site detection of chironomids, supporting early warning, and ecological monitoring in freshwater environments. Abstract BACKGROUND Freshwater chironomid midges pose a significant threat to drinking water quality and ...
Kyuhyeong Kim, Bhavya Kachiprath, In‐Cheol Yeo, Kyu‐Young Shim, Ihn‐Sil Kwak, Chang‐Bum Jeong   +5 more
wiley   +1 more source

Hutchinson Gilford Progeria Syndrome: A Therapeutic Approach via Adenoviral Delivery of CRISPR/cas Genome Editing System [PDF]

, 2014
Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare human genetic disease caused by mutations in the LMNA gene. LMNA codes for structural components of the nuclear lamina. Alterations of nuclear lamina lead to a very variable class of diseases known as
ARANCIO, Walter, Genovese, SI, GIORDANO, Carla, PIZZOLANTI, Giuseppe   +3 more
core   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes [PDF]

, 2017
Prokaryotes encode various host defense systems that provide protection against mobile genetic elements. Restriction-modification (R-M) and CRISPR-Cas systems mediate host defense by sequence specific targeting of invasive DNA.
Anders, C., Brouns, S.J.J., Dickman, M.J., Houkes, J., Jinek, M., Kunne, T., Lochs, S.J.A., Mohanraju, P., Swarts, D.C., van der Oost, J., Vlot, M., Zheng, P.   +11 more
core   +3 more sources