Results 51 to 60 of about 38,906 (120)

A Susceptible Cell‐Selective Delivery (SCSD) of mRNA‐Encoded Cas13d Against Influenza Infection

Advanced Science, Volume 12, Issue 9, March 6, 2025.
A CRISPR/Cas13d system is designed for broad‐spectrum targeting of IAV, incorporating Cas13d mRNA and a tandem crRNA specific for conserved regions of the viral PA, NP, and M gene segments, respectively. This CRISPR/Cas13d system is delivered by a Susceptible Cell Selective Delivery (SCSD) system which targets sialic acid receptors and shows effective ...
Zhuanli Wu, Chengcheng Zhao, Hui Ai, Zhen Wang, Mingyue Chen, Yanli Lyu, Qi Tong, Litao Liu, Honglei Sun, Juan Pu, Ran Zhang, Xiaoxiang Hu, Jinhua Liu, Xiaowei Ma, Yipeng Sun   +14 more
wiley   +1 more source

Efficient Gene Editing of CART Cells with CRISPR-Cas12a for Enhanced Antitumor Efficacy

, 2020
Chimeric antigen receptor T (CART) cells are engineered with an artificial receptor which redirects T cells to recognize cancer cells expressing a particular surface antigen.
Elizabeth L. Siegler, Brandon W. Simone, R. Sakemura, Erin E Tapper, Paulina Horvei, Michelle J. Cox, M. Hefazi, C. Roman, Ismail Can, Kendall J. Schick, M. Ruff, S. Ekker, S. Kenderian   +12 more
semanticscholar   +1 more source

High Nutritional Conditions Influence Feeding Plasticity in Pristionchus pacificus and Render Worms Non‐Predatory

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Volume 344, Issue 2, Page 94-111, March 2025.
Some nematodes exhibit mouth‐form polyphenism resulting in different feeding strategies including predation. In this study, Piskobulu and co‐workers investigate the influence of glucose supplementation on mouth‐form expression and find that higher glucose levels render worms non‐predatory.
Veysi Piskobulu, Marina Athanasouli, Hanh Witte, Christian Feldhaus, Adrian Streit, Ralf J. Sommer   +5 more
wiley   +1 more source

Prediction and Validation of Native and Engineered Cas9 Guide Sequences.

Cold Spring Harbor Protocols, 2016
Cas9-based technologies rely on native elements of Type II CRISPR-Cas bacterial immune systems, including the trans-activating CRISPR RNA (tracrRNA), CRISPR RNA (crRNA), Cas9 protein, and protospacer-adjacent motif (PAM).
A. Briner, E. D. Henriksen, R. Barrangou
semanticscholar   +1 more source

'Splice-at-will' Cas12a crRNA engineering enabled direct quantification of ultrashort RNAs. [PDF]

Nucleic Acids Res
Fei X, Lei C, Ren W, Liu C.
europepmc   +1 more source

Regulation of CRISPR trans-cleavage activity by an overhanging activator. [PDF]

Nucleic Acids Res
Yin N, Yu H, Zhang L, Luo F, Wang W, Han X, He Y, Zhang Y, Wu Y, Pu J, Feng T, Yang G, Chen T, Xie G.   +13 more
europepmc   +1 more source

DNA target binding-induced pre-crRNA processing in type II and V CRISPR-Cas systems. [PDF]

Nucleic Acids Res
Chen J, Lin X, Xiang W, Chen Y, Zhao Y, Huang L, Liu L.   +6 more
europepmc   +1 more source

Exploring the effect of activator topology on CRISPR-Cas12a trans-cleavage activity. [PDF]

Nucleic Acids Res
Zhu Z, Li X, Ding L, Wu T.
europepmc   +1 more source

Expanding Cas12a Activity Control with an RNA G-Quadruplex at the 5' end of CRISPR RNA. [PDF]

Adv Sci (Weinh)
Huang W, Wang J, Wang C, Liu Y, Li W, Chen Q, Zhai J, Xiang Z, Liu C.   +8 more
europepmc   +1 more source

Molecular insights and rational engineering of a compact CRISPR-Cas effector Cas12h1 with a broad-spectrum PAM. [PDF]

Signal Transduct Target Ther
Zheng W, Li H, Liu M, Wei Y, Liu B, Li Z, Xiong C, Huang S, Hu C, Ouyang S.   +9 more
europepmc   +1 more source