Results 61 to 70 of about 43,663 (164)

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa, Mamata Karmacharya, Hyunmin Choi, Sumit Kumar, Yoon‐Kyoung Cho   +4 more
wiley   +1 more source

Cas12Fold Accurately Predicts Cas12 Nuclease Structures to Enable Structure‐based Genome‐editing Engineering

Small Structures, EarlyView.
Cas12Fold, a deep‐learning framework tailored to Cas12 nucleases, uses evolutionary signals and iterative structure‐based alignment to resolve conformational complexity. This method outperforms general models in predicting key domains and states, accurately modeling enzymes such as Casλ.
Feng Xu, Zilong Zhao, Chang Liu, Meixia Yu, Ke Li, Yilin Jing, Peiyang Li, Beibei Xin, Jian Chen, Lizhu E, Jinsheng Lai, Zhijia Yang, Hainan Zhao   +12 more
wiley   +1 more source

STIM2?? is upregulated by insulin signaling and involved in adipogenesis [PDF]

, 2015
Department of Biological SciencesCalcium ion play an important role in lipid formation and adipogenesis. The STIM1 and STIM2 are endoplasmic reticulum Ca2+ sensors which activate and regulate Orai, store operated calcium channel. I am interested in stim2?
Kim, Mi Ri
core  

Heritable genome editing in C. elegans via a CRISPR-Cas9 system [PDF]

, 2014
CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C.
Calarco, John A., Church, George M., Colaiácovo, Monica P., Esvelt, Kevin M., Friedland, Ari E., Tzur, Yonatan B.   +5 more
core   +1 more source

Mouse genome engineering uncovers 18 genes dispensable for male reproduction

Andrology, EarlyView.
Abstract Background Male infertility is an intricate multifactorial disease involving the interplay between genetic and environmental factors. Genetic anomalies account for more than 15% of all male infertility cases; however, diagnosing them exhibits enormous challenges due to variable symptomatic presentations and limited knowledge of gene functions.
Hsin‐Yi Chang, Yonggang Lu, Kaito Yamamoto, Jiang Sun, Keisuke Shimada, Yuki Hiradate, Yoshitaka Fujihara, Masahito Ikawa   +7 more
wiley   +1 more source

DNA-guided CRISPR/Cas12 for RNA targeting

Research Square
CRISPR-Cas nucleases are transforming genome editing, RNA editing, and diagnostics but have been limited to RNA-guided systems. We present ΨDNA, a DNA-based guide for Cas12 enzymes, engineered for specific and efficient RNA targeting. ΨDNA mimics a crRNA
P. Jain, Carlos Orosco, Santosh R. Rananaware, Boyu Huang, Michael P Hanna, M. Ahmadimashhadi, Jordan G Lewis, Michael P Baugh, August P Bodin, Sarah J Flannery, Ian Langue, Zoe R Fang, Vedant N Karalkar, Katelyn S. Meister   +13 more
semanticscholar   +2 more sources

How the other half lives: CRISPR-Cas's influence on bacteriophages

, 2017
CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers.
AE Briner, AF Andersson, BR Levin, C Pourcel, CL Sun, D Bhaya, E Pennisi, E Scarpellini, EV Koonin, FS Berezovskaya, GW Tyson, H Deveau, H Deveau, I Yosef, IN Berezovsky, J He, J Iranzo, J Mahony, JF Heidelberg, JM Peters, JO Haerter, KD Seed, KL Maxwell, LA Marraffini, LM Childs, M Sebaihia, P Han, P Horvath, P Horvath, PD Hsu, R Barrangou, R Lillestøl, R Sorek, S Al-Attar, S Minot, W Jiang   +35 more
core   +1 more source

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases [PDF]

, 2014
Current antibiotics tend to be broad spectrum, leading to indiscriminate killing of commensal bacteria and accelerated evolution of drug resistance.
AA Gomaa, AP Desbois, B Mnif, C Westwater, CJ Paddon, CT Chung, D Bikard, D Dong, D Pérez-Mendoza, DG Gibson, DJ Dwyer, E Deltcheva, F Duan, F Hayes, GA Jacoby, JB Kaper, JE Garneau, JJ Williams, JK Rasheed, JK Rasheed, JL Sonnenburg, JM Pennington, KD Seed, L Chasteen, M Jinek, Mark Mimee, P Mali, P Nordmann, R Barrangou, R Edgar, R Lutz, RB Vercoe, Robert J Citorik, RS Sikorski, S Datta, Timothy K Lu, TK Lu, TK Lu, W Jiang   +38 more
core   +1 more source

Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice

Andrology, EarlyView.
ABSTRACT Background Sperm motility is essential for male fertility, and its regulation is dependent on the structural integrity of the axoneme. The axoneme consists of a conserved “9+2” microtubule arrangement and is supported by microtubule inner proteins. However, the functional significance of many microtubule inner proteins remains unclear.
Haoting Wang, Anh Hoang Pham, Mengjiao Luo, Masahito Ikawa, Haruhiko Miyata   +4 more
wiley   +1 more source

CRISPR-Cas systems in prokaryotes [PDF]

, 2015
Prokaryotic organisms possess numerous strategies that enable survival in hostile conditions. Among others, these conditions include the invasion of foreign nucleic acids such as bacteriophages and plasmids.
Burmistrz, Michał, Pyrć, Krzysztof
core   +1 more source