Results 71 to 80 of about 43,663 (164)

A functional type II-A CRISPR–Cas system from Listeria enables efficient genome editing of large non-integrating bacteriophage

Nucleic Acids Research, 2018
CRISPR–Cas systems provide bacteria with adaptive immunity against invading DNA elements including bacteriophages and plasmids. While CRISPR technology has revolutionized eukaryotic genome engineering, its application to prokaryotes and their viruses ...
Mario Hupfeld, Despoina Trasanidou, Livia Ramazzini, J. Klumpp, M. Loessner, S. Kilcher   +5 more
semanticscholar   +1 more source

Cas9 beyond CRISPR – SUMOylation, effector‐like potential and pathogenic adaptation

The FEBS Journal, EarlyView.
This Viewpoint explores the possibility that Cas9, beyond its canonical role in CRISPR immunity, may act as a host‐modulating effector during infection by Cas9‐encoding bacteria. Such a nucleomodulin‐like function could be influenced by eukaryotic post‐translational modifications, particularly SUMOylation, with potential implications for host–pathogen ...
Umut Sahin
wiley   +1 more source

Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line [PDF]

, 2016
This project was carried out during CD’s BBSRC Eastbio PIPS placement at Marine Scotland. The authors are grateful to Dr Milena Monte (University of Aberdeen) for help with the FACS analysis.
Boudinot, Pierre, Collet, Bertrand, Dehler, Carola E., Martin, Samuel A. M.   +3 more
core   +2 more sources

CRISPR‐Enhanced RAA‐SHERLOCK Assay for Point‐of‐Care Detection of Cyprinid Herpesvirus‐3: Development, Validation and Clinical Application

Journal of Fish Diseases, EarlyView.
ABSTRACT Koi herpesvirus disease (KHVD), caused by Cyprinid herpesvirus‐3 (CyHV‐3), poses a significant threat to global aquaculture due to its high mortality rates and economic impact. Current diagnostic methods, such as PCR, are limited by equipment dependency and procedural complexity, hindering point‐of‐care (POC) applications.
Hatem Soliman, Naveed Akram, Mona Saleh
wiley   +1 more source

An Agrobacterium‐delivered CRISPR/Cas9 system for targeted mutagenesis in sorghum

Plant Biotechnology Journal, 2019
Clustered regularly interspaced short palindromic repeat/CRISPR-associated Cas9 (CRISPR/Cas9) systems of bacteria and archaea have engineered for genome editing in eukaryotic genomes.
S. N. Char, Jialu Wei, Qi Mu, Xianran Li, Zhanyuan J. Zhang, Jianming Yu, Bing Yang   +6 more
semanticscholar   +1 more source

Symbiosis‐associated UMAMIT transporters required for establishing efficient nitrogen fixation in Medicago truncatula

New Phytologist, EarlyView.
Summary To address a critical gap in understanding amino acid transport in legume–Rhizobium symbiosis, we investigated the role of symbiosis‐associated USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTERS (UMAMITs) in Medicago truncatula nodulation.
Courtney S. Winning, María Isabel Rubia, Wei Liu, Daniel Bronitt, Ángel María Zamarreño, José María García‐Mina, Penelope Smith, Estíbaliz Larrainzar, Michael A. Djordjevic   +8 more
wiley   +1 more source

Editing streptomycete genomes in the CRISPR/Cas9 age [PDF]

, 2019
This article aims to highlight advantages, drawbacks and issues that users should consider when implementing the use of CRISPR/Cas9-tools for genome editing in streptomycetes, the most prolific source of antimicrobial natural products to date.
Alberti, Fabrizio, Corre, Christophe
core   +1 more source

CRISPR/Cas‐Mediated Gene Editing in Plant Immunity and Its Potential for the Future Development of Fungal, Oomycete, and Bacterial Pathogen‐Resistant Pulse Crops

Plant, Cell &Environment, EarlyView.
ABSTRACT Pulses provide myriad health benefits and are advantageous in an environmental context as a result of their leguminous nature. However, phytopathogenic fungi, oomycetes and bacteria pose a substantial threat to pulse production, at times leading to crop failure.
Stacy D. Singer, Mohammed M. Mukthar, Udaya Subedi, Hari Poudel, Guanqun Chen, Nora Foroud, Syama Chatterton   +6 more
wiley   +1 more source

Structure and Engineering of Francisella novicida Cas9 [PDF]

, 2016
Summary The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing.
Adams, Anders, Briner, Chylinski, Cong, Cowtan, Crooks, Deltcheva, Deveau, Emsley, Evans, Feng Zhang, Fonfara, Garneau, Gasiunas, Hiroshi Nishimasu, Hisato Hirano, Hsu, Hsu, Izuho Hatada, Jiang, Jiang, Jinek, Jinek, Jonathan S. Gootenberg, Kabsch, Kim, Kleinstiver, Kleinstiver, Lin, Mali, Mika Kimura, Mojica, Nishimasu, Nishimasu, Omar O. Abudayyeh, Osamu Nureki, Patrick D. Hsu, Ran, Ryuichiro Ishitani, Sampson, Sternberg, Sung, Takanori Nakane, Takuro Horii, Woo, Zetsche, Zuris   +47 more
core   +1 more source

Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next‐Generation Diagnostics and Biosensing

Advanced Science, Volume 13, Issue 2, 9 January 2026.
The advent of aptamers has highlighted their potential as alternatives to antibodies, overcoming limitations of structural instability and production cost. However, conventional approaches such as SELEX remain slow and labor‐intensive. This review examines recent advances in aptamer engineering, emphasizing in vitro and AI‐driven in silico strategies ...
John V. L. Nguyen, Ahlem Meziadi, Cyrill Brunner, Maureen McKeague, Lidija Malic, Maryam Tabrizian   +5 more
wiley   +1 more source