Iron Deprivation in Synechocystis : Inference of Pathways, Non-coding RNAs, and Regulatory Elements from Comprehensive Expression Profiling [PDF]
, 2012 Iron is an essential cofactor in many metabolic reactions. Mechanisms controlling iron homeostasis need to respond rapidly to changes in extracellular conditions, but they must also keep the concentration of intracellular iron under strict control to ...
Barreira, L +6 more
core +2 more sources
Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endonuclease*
Journal of Biological Chemistry, 2014 Background: The Cas9 RNA-guided endonuclease has been adapted for genome manipulation and regulation. Results: We have characterized target recognition and cleavage by Streptococcus thermophilus LMG18311 Cas9. Conclusion: The two nuclease domains of Cas9
Hongfan Chen, Ji-Sun Choi, S. Bailey
semanticscholar +1 more source
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems [PDF]
, 2013 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic acids.
Aach, John Dennis +5 more
core +1 more source
Advances in biotechnology: genomics and genome editing [PDF]
, 2017 Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso +4 more
core +1 more source
CRISPR/Cas9-mediated mutagenesis of CAROTENOID CLEAVAGE DIOXYGENASE 8 in tomato provides resistance against the parasitic weed Phelipanche aegyptiaca. [PDF]
, 2019 Broomrapes (Phelipanche aegyptiaca and Orobanche spp.) are obligate plant parasites that cause extreme damage to crop plants. The parasite seeds have strict requirements for germination, involving preconditioning and exposure to specific chemicals ...
Aly, Radi +8 more
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Engineering CRISPR guide RNA riboswitches for in vivo applications [PDF]
, 2019 CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and ...
Galizi, Roberto, Jaramillo, Alfonso
core +1 more source
The TGFβ type I receptor TGFβRI functions as an inhibitor of BMP signaling in cartilage. [PDF]
, 2019 The type I TGFβ receptor TGFβRI (encoded by Tgfbr1) was ablated in cartilage. The resulting Tgfbr1 Col2 mice exhibited lethal chondrodysplasia. Similar defects were not seen in mice lacking the type II TGFβ receptor or SMADs 2 and 3, the intracellular ...
Baek, Jongseung +7 more
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Overview of the CRISPR-Cas system and design of sgRNAs ("single guide RNAs") for genomic DNA editing in yeast [PDF]
, 2017 Traballo fin de grao (UDC.CIE). Bioloxía. Curso 2016/2017[Resumen] El descubrimiento de los sistemas CRISPR-Cas y concretamente del sistema CRISPR-Cas9 ha supuesto una revolución en el campo de la ingeniería genómica y la biotecnología, puesto que ...
Pardo Piñón, Marta
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Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes [PDF]
, 2018 The Cas9 endonuclease can be programmed by guide RNA to introduce sequence-specific breaks in genomic DNA. Thus, Cas9-based approaches present a range of novel options for genome manipulation and precision editing. African trypanosomes are parasites that
A Paix +46 more
core +2 more sources
Développement du système CRISPR/Cas9 pour l'ingénierie du génome ciblé chez Pseudomonas putida [PDF]
, 2018 Ce travail avait pour but de développer un nouveau système permettant de modifier le génome de Pseudomonas putida KT2440.
Bélaz, Eléa, Schmid, Sergio
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