Results 351 to 360 of about 968,391 (388)
Some of the next articles are maybe not open access.
New Micellar Transfection Agents
Langmuir, 2014Two novel micelle-forming amino-functionalized lipids (OT6 and TT6) bearing two alkyl chains connected to a large positively charged hexavalent headgroup, which might be interesting polynucleotide transferring agents with the advantage of an easy and reproducible production of micelle dispersions, have been characterized.
Wölk, C. +9 more
openaire +3 more sources
Keratinocyte Transient Transfections
2004The goal of this chapter is to give the reader a concise and easy-to-follow guide to proven transient transfection techniques for primary strains and continuous lines of keratinocytes. The emphasis is on readily available and inexpensive resources that also allow for repeatability and adaptability to scale, experimental conditions, and sample ...
Anthony M, Flores, Brian J, Aneskievich
openaire +2 more sources
Current Protocols in Neuroscience, 1997
AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
openaire +2 more sources
AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
openaire +2 more sources
Transfection of Difficult-to-Transfect Primary Mammalian Cells
2011Primary cells are a valuable tool for researchers and are often preferred over transformed or immortalized cell lines since they are biologically more relevant and resemble the in vivo situation much closer. Unfortunately, efficient gene transfer in primary cells is still limited.
Oliver, Gresch, Ludger, Altrogge
openaire +2 more sources
Nihon rinsho. Japanese journal of clinical medicine, 1997
Transfection is a method to introduce DNA into mammalian cells. Calcium phosphate-mediated transfection is well used as one of the methods for transfection. The basic protocol is simple and does not require the elaborated instruments. It has been applied to introduce the heterogeneous DNA for integration into and expression from the chromosome.
openaire +1 more source
Transfection is a method to introduce DNA into mammalian cells. Calcium phosphate-mediated transfection is well used as one of the methods for transfection. The basic protocol is simple and does not require the elaborated instruments. It has been applied to introduce the heterogeneous DNA for integration into and expression from the chromosome.
openaire +1 more source
Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers.
Advanced Drug Delivery Reviews, 2008Igor I. Slowing +3 more
semanticscholar +1 more source
Exploring polyethylenimine‐mediated DNA transfection and the proton sponge hypothesis
Journal of Gene Medicine, 2005Akin Akinc +3 more
semanticscholar +1 more source
Optimized PEI‐based Transfection Method for Transient Transfection and Lentiviral Production
Current Protocols in Chemical Biology, 2017Shaozhe Yang +4 more
semanticscholar +1 more source