Forensic trace DNA analysis to answer activity-level questions in a realistic-scenario activity study based upon the case of Idaho v Bryan C. Kohberger. [PDF]
Alviar A, Wickenheiser R, Hall A.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Thermo-hydraulic performance analysis of a horizontal slinky-type geothermal heat exchanger under turbulent flow vonditions. [PDF]
Hsu CY +9 more
europepmc +1 more source
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Lipid traffic: an enigmatic alliance between ORPs and a TMEM16-like protein at membrane contact sites. [PDF]
Fabbre A, Drin G.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Breastfeeding at discharge or transfer from the maternity hospital: results from the German Perinatal Statistics 2021. [PDF]
Brettschneider AK +4 more
europepmc +1 more source
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Intercellular mitochondrial transfer in melanoma progression and therapeutic resistance: mechanisms and targeting potential. [PDF]
Yan Q, Sun T, Chen K, Hu H.
europepmc +1 more source

