Results 31 to 40 of about 538,017 (259)

Characterization of UVA-Induced Alterations to Transfer RNA Sequences

Biomolecules, 2020
Ultraviolet radiation (UVR) adversely affects the integrity of DNA, RNA, and their nucleoside modifications. By employing liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based RNA modification mapping approaches, we identified the transfer RNA (
Congliang Sun, Patrick A. Limbach, Balasubrahmanyam Addepalli   +2 more
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Complete mitochondrial genome of Sinogastromyzon szechuanensis (Teleostei, Cypriniformes, Balitoridae) obtained using next-generation sequencing

Mitochondrial DNA. Part B. Resources, 2021
The complete mitogenome sequence of Sinogastromyzon szechuanensis was obtained using next generation sequencing and confirmed via overlap-PCR method.
Wei Li, Xiaggojiang Chen, Mengmeng Zheng, Yin Wang, Xingchen Zhou, Haiyang Li, Guolong Zhou, Bichun Li   +7 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles

Molecular Therapy: Methods & Clinical Development, 2015
RNA delivery is an attractive strategy to achieve transient gene expression in research projects and in cell- or gene-based therapies. Despite significant efforts investigating vector-directed RNA transfer, there is still a requirement for better ...
Anne Prel, Vincent Caval, Régis Gayon, Philippe Ravassard, Christine Duthoit, Emmanuel Payen, Leila Maouche-Chretien, Alison Creneguy, Tuan Huy Nguyen, Nicolas Martin, Eric Piver, Raphaël Sevrain, Lucille Lamouroux, Philippe Leboulch, Frédéric Deschaseaux, Pascale Bouillé, Luc Sensébé, Jean-Christophe Pagès   +17 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Analysis of extracellular RNA by digital PCR

Frontiers in Oncology, 2014
The transfer of extracellular RNA is emerging as an important mechanism for intracellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to ...
Kenji eTakahashi, Irene K Yan, Chaeyoung eKim, Jungsu eKim, Tushar ePatel   +4 more
doaj   +1 more source

Smuggling on the Nanoscale—Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo

Pharmaceutics, 2023
The efficient and biocompatible transfer of nucleic acids into mammalian cells for research applications or medical purposes is a long-standing, challenging task.
Marco Hoffmann, Sven Gerlach, Masanari Takamiya, Samar Tarazi, Nils Hersch, Agnes Csiszár, Ronald Springer, Georg Dreissen, Hanno Scharr, Sepand Rastegar, Tanja Beil, Uwe Strähle, Rudolf Merkel, Bernd Hoffmann   +13 more
doaj   +1 more source