Results 91 to 100 of about 155,620 (276)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Cerebrospinal fluid and blood profiles of transfer RNA fragments show age, sex and Parkinson’s disease-related changes [PDF]

, 2022
Iddo Paldor, Nimrod Madrer, Shani Vaknine, Dana Shulman, David Greenberg, Hermona Soreq   +5 more
openalex   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Cytosine-5 RNA methylation links protein synthesis to cell metabolism.

PLoS Biology, 2019
Posttranscriptional modifications in transfer RNA (tRNA) are often critical for normal development because they adapt protein synthesis rates to a dynamically changing microenvironment.
Nikoletta A Gkatza, Cecilia Castro, Robert F Harvey, Matthias Heiß, Martyna C Popis, Sandra Blanco, Susanne Bornelöv, Abdulrahim A Sajini, Joseph G Gleeson, Julian L Griffin, James A West, Stefanie Kellner, Anne E Willis, Sabine Dietmann, Michaela Frye   +14 more
doaj   +1 more source

Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients

, 2021
Li Meng, Long Jiang, Jian Chen, Hongjin Ren, Zhiqin Gao, Fei Wu, Yiyang Wen, Lianjuan Yang   +7 more
openalex   +2 more sources

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Transfer RNA‐derived fragment 5′tRF‐Gly promotes the development of hepatocellular carcinoma by direct targeting of carcinoembryonic antigen‐related cell adhesion molecule 1

, 2022
Dekai Liu, Chengdong Wu, Jingjie Wang, Lufei Zhang, Zhongquan Sun, Shihong Chen, Yuan Ding, Weilin Wang   +7 more
openalex   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

Differential Expression Profiles and Function Prediction of Transfer RNA‐Derived Fragments in High‐Grade Serous Ovarian Cancer [PDF]

, 2021
Buze Chen, Sicong Liu, Haihong Wang, Guilin Li, Xiaoyuan Lu, Hao Xu   +5 more
openalex   +1 more source