Results 291 to 300 of about 3,618,105 (334)
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Environmental Science and Technology, 2022
To advance the understanding of antibiotic resistance propagation from wastewater treatment plants, it is important to elucidate how different effluent disinfection processes affect the dissemination of predominantly extracellular antibiotic resistance ...
Qing-bin Yuan +7 more
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To advance the understanding of antibiotic resistance propagation from wastewater treatment plants, it is important to elucidate how different effluent disinfection processes affect the dissemination of predominantly extracellular antibiotic resistance ...
Qing-bin Yuan +7 more
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Bacterial Transformation (Kushner Method)
2003The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells competent, which simply means they are now able to take up DNA from the solution.
J W, Dale, P J, Greenaway
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Bacterial transformation of terpenoids
Russian Chemical Reviews, 2014Data on the bacterial transformation of terpenoids published in the literature in the past decade are analyzed. Possible pathways for chemo-, regio- and stereoselective modifications of terpenoids are discussed. Considerable attention is given to new technological approaches to the synthesis of terpenoid derivatives suitable for the use in the perfume ...
V V Grishko +2 more
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2003
If one consults any genetics textbook written before the mid-1970s, one will find much on the transformation of such bacterial species as Bacillus subtilis, but no mention of Escherichia coli. The method now most generally used for introducing DNA into E.
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If one consults any genetics textbook written before the mid-1970s, one will find much on the transformation of such bacterial species as Bacillus subtilis, but no mention of Escherichia coli. The method now most generally used for introducing DNA into E.
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Mechanism of Bacterial Transformation and Transfection
1974Publisher Summary This chapter discusses that in spite of the large amount of information on the physiology of the competent cell, including the production of competence factors in a number of transformation systems and an additional protein that must be synthesized to permit irreversible binding of DNA by D.
N K, Notani, J K, Setlow
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"Steady-state" competence for bacterial transformation
Canadian Journal of Microbiology, 1968The cells of Bacillus subtilis, grown in minimal media, are known to become competent for transformation for a short period at a specific phase of ceil growth. In the present work the cells (strain 168 ind−) were grown in continuous culture (chemostat; glucose limiting, generation time 4 h, 37 °C).
H H, Eichhorn, S, Arikawa, S, Zamenhof
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Impact of heat shock step on bacterial transformation efficiency
Molecular Biology Research Communications, 2016CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the ...
Maral Rahimzadeh +4 more
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A simple and rapid method of bacterial transformation
Journal of Microbiological Methods, 2010Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive,
Gottfried, Wilharm +5 more
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Current Status of Bacterial Transformations
1955Publisher Summary When it became clear that a number of plant viruses are crystallizable macromolecules composed of ribonucleic acid and protein, it was possible to attribute to a particular sort of macromolecule the striking biological property of autoreproduction.
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Strategies for the development of bacterial transformation systems
Biochimie, 1988An effective transformation system is a prerequisite for facile genetic manipulation of bacteria. Bacteria may be naturally competent for transformation or may be treated with various agents, such as Tris buffers or divalent metal ions, to induce competence.
A, Mercenier, B M, Chassy
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