Results 321 to 330 of about 2,858,382 (405)
The Plant Journal, 2013 Reverse genetics approaches have contributed enormously to the elucidation of gene functions in plastid genomes and the determination of structure-function relationships in chloroplast multiprotein complexes.
Katharina Krech +5 more
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Whole genome sequencing and the transformation of C. elegans forward genetics
Methods, 2014 Patrick J Hu
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Pathogenesis of follicular lymphoma: genetics to the microenvironment to clinical translation
British Journal of Haematology, 2021Follicular lymphoma (FL) represents a heterogeneous disease both clinically and biologically. The pathognomonic t(14;18) translocation can no longer be thought of as the primary genetic driver, with increasing recognition of the biological relevance of ...
E. Kumar, Lucy Pickard, J. Okosun
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Genetic transformation of fungi
The International Journal of Developmental Biology, 2017Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely ...
Chunmei Chen +7 more
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2011
Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Tubercle, 1989
Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
Barry R. Bloom +2 more
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Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
Barry R. Bloom +2 more
openaire +3 more sources
Genetic transformation of mycobacteria [PDF]
Trends in Microbiology, 1993 Recent advances in methods for introducing DNA into both fast- and slow-growing species of mycobacteria have stimulated the construction of a variety of cloning vectors and the development of a versatile genetic system. These tools will facilitate studies of mycobacterial pathogenicity, antibiotic action and drug resistance, and lead to more effective ...
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Genetic Transformation of Switchgrass
2009Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used
Zeng-Yu Wang, Yajun Xi, Yaxin Ge
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The enzymes, regulation, and genetics of bile acid synthesis.
Annual Review of Biochemistry, 2003The synthesis and excretion of bile acids comprise the major pathway of cholesterol catabolism in mammals. Synthesis provides a direct means of converting cholesterol, which is both hydrophobic and insoluble, into a water-soluble and readily excreted ...
D. Russell
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Genetics of drug transformation
Clinical Biochemistry, 1986Biotransformations of drugs are controlled or strongly affected by genetic factors. During the past few years several genetic deficiencies of drug-metabolizing reactions catalyzed by members of the family of cytochrome P-450 were observed. Choice of the appropriate drug to study and attention to urinary metabolites have been the essential ingredients ...
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