A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Self-supervised transformers predict dynamics of object-based attention in humans
, 2023 Hossein Adeli +3 more
openalex +1 more source
OCTraN: 3D Occupancy Convolutional Transformer Network in Unstructured Traffic Scenarios [PDF]
, 2023 Aditya Nalgunda Ganesh +4 more
openalex +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
A Fuzzy Evaluation Method of Power Transformer Insulation Health State Based on Multi-weight Fusion
, 2022 Jifang Li +3 more
openalex +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Evaluation of climate prediction models in Yunnan, China: traditional methods and AI approaches. [PDF]
Sci RepZhao J, Zhao F, Deng H.
europepmc +1 more source
Evaluation Method of 500kV Transformer Bushing Insulation State Based on Active Power Loss
, 2022 Zhaoliang Gu +7 more
openalex +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source