In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
A State Dependent Regime Switching Model of Dynamic Correlations [PDF]
Replaced with revised version of paper 07/29/09.dynamic correlations, regime switching, state dependent probabilities, thresholds, spillovers, Research Methods/ Statistical Methods,
Tejeda, Hernan A. +2 more
core
Regime shifts and climate responses of alpine grasslands in Gannan Prefecture, China. [PDF]
Hou Q +7 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Ageing of Oxygen-Plasma-Treated Polytetrafluoroethylene Surfaces: Revealing a Novel Link Between Morphological Evolution and Wettability. [PDF]
Maryam R, Barni R, Roman HE, Riccardi C.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Variational modeling and numerical simulations for evaporating thin droplets and coffee-ring effect. [PDF]
Li Y, Zhao Q, Qian T.
europepmc +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Beyond emissions: a stochastic cusp-logit analysis of environmental cost regimes in BRICS. [PDF]
Mehta D, Patel N, Abdi AH.
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source

