Results 131 to 140 of about 8,762 (179)
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Inhibition of transketolase by analogues of the coenzyme
Biochemical and Biophysical Research Communications, 1971Abstract The effect of thiamine, thiamine monophosphate, pyrophosphate and thiazole pyrophosphate on the enzymatic activity of transketolase has been studied. All these compounds have been proved to inhibit the enzyme by competing with the coenzyme (thiamine pyrophosphate) for apotransketolase.
G A, Kochetov +2 more
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Erythrocyte transketolase activity in uremia
Clinica Chimica Acta, 1980We performed a study concerning the activity of erythrocyte transketolase and thiamine metabolism in 73 uremic patients with or without neuropathy and 67 normal control subjects. Although the total vitamin B1 level in whole blood was high in the uremic patients, the transketolase activity in the hemolysate and the thiamine pyrophosphate effect on it ...
M, Kuriyama +4 more
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Analysis of Blood Transketolase Activity
American Journal of Clinical Pathology, 1971The determination of blood transketolase (TK) activity is based on the incubation of lysed erythrocytes with and without added thiamine pyrophosphate (TPP). The increased TK activity resulting from the addition of TPP coenzyme is inversely related to thiamine depletion.
M F, Massod, S L, McGuire, K R, Werner
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Isolation and properties of human transketolase
Biochemistry (Moscow), 2010Recombinant human (His)(6)-transketolase (hTK) was obtained in preparative amounts by heterologous expression of the gene encoding human transketolase in Escherichia coli cells. The enzyme, isolated in the form of a holoenzyme, was homogeneous by SDS-PAGE; a method for obtaining the apoenzyme was also developed.
L E, Meshalkina +4 more
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Donor substrate regulation of transketolase
European Journal of Biochemistry, 2004The influence of substrates on the interaction of apotransketolase with thiamin diphosphate was investigated in the presence of magnesium ions. It was shown that the donor substrates, but not the acceptor substrates, enhance the affinity of the coenzyme either to only one active center of transketolase or to both active centers, but to different ...
Olga A, Esakova +4 more
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Sulfhydryl groups and transketolase activity
Biochemical and Biophysical Research Communications, 1966Abstract Transketolase, an enzyme of pentose cycle, catalyzes the reaction of glycolaldehyde transport from the xylulose-5-phosphate to the ribose-5-phosphate with the formation of sedoheptulose-7-phosphate. There is not much known about this enzyme, especially in respect to its functional groups.
G A, Kochetov, F G, Lutovinova
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Functional Flexibility of the Transketolase Molecule
Biochemistry (Moscow), 2001Transketolase is the simplest representative of the thiamine diphosphate-dependent enzymes. It was the first of these enzymes for which X-ray analysis was performed. Based on the data of X-ray studies and using the mutagenesis technique, the nature of functional groups of the enzyme involved in the interaction with substrates and cofactors and in the ...
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Anaemia and Erythrocyte Transketolase Activity
Acta Haematologica, 2009Erythrocyte transketolase activity is raised in megaloblastic anaemia due to vitamin B<sub>12</sub> deficiency and inversely related to haemoglobin concentration. It si usually normal in cases of megaloblastic anaemia due to folate deficiency though sufficient exceptions occur to invalidate the use of red cell transketolase assay in ...
D G, Wells, V, Marks
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