Results 131 to 140 of about 8,654 (179)

Enhancement of the Substrate Scope of Transketolase

ChemBioChem, 2012
AbstractTo enhance the activity of transketolase towards nonphosphorylated substrates and enlarge the scope of its substrates, notably to long polyol aldehyde acceptors (D‐ribose or D‐glucose), a rational design‐supported evolution strategy was applied.
Sanjib Kumar Karmee, Isabel Arends, Ulf Hanefeld   +2 more
exaly   +3 more sources

The Catalytic Mechanism of Human Transketolase

ChemPhysChem, 2019
AbstractWe have computationally determined the catalytic mechanism of human transketolase (hTK) using a cluster model approach and density functional theory calculations. We were able to determine all the relevant structures, bringing solid evidences to the proposed experimental mechanism, and to add important detail to the structure of the transition ...
Fabiola E Medina, Maria João Ramos, Tiziana Marino   +2 more
exaly   +3 more sources

Inhibition of transketolase by hexacyanoferrate(III)

Biochemistry (Moscow), 2010
The effect of hexacyanoferrate(III) on the catalytic activity of transketolase has been studied. This oxidant inactivates only one of two active sites of the enzyme, the one with a higher affinity to the coenzyme (thiamine diphosphate). The second active site does not lose its catalytic activity.
V A, Yurshev, I A, Sevostyanova, O N, Solovjeva, G A, Kochetov   +3 more
openaire   +2 more sources

Sulfhydryl groups and transketolase activity

Biochemical and Biophysical Research Communications, 1966
Abstract Transketolase, an enzyme of pentose cycle, catalyzes the reaction of glycolaldehyde transport from the xylulose-5-phosphate to the ribose-5-phosphate with the formation of sedoheptulose-7-phosphate. There is not much known about this enzyme, especially in respect to its functional groups.
G A, Kochetov, F G, Lutovinova
openaire   +2 more sources

Inhibition of transketolase by analogues of the coenzyme

Biochemical and Biophysical Research Communications, 1971
Abstract The effect of thiamine, thiamine monophosphate, pyrophosphate and thiazole pyrophosphate on the enzymatic activity of transketolase has been studied. All these compounds have been proved to inhibit the enzyme by competing with the coenzyme (thiamine pyrophosphate) for apotransketolase.
G A, Kochetov, A E, Izotova, L E, Meshalkina   +2 more
openaire   +2 more sources

Erythrocyte transketolase activity in uremia

Clinica Chimica Acta, 1980
We performed a study concerning the activity of erythrocyte transketolase and thiamine metabolism in 73 uremic patients with or without neuropathy and 67 normal control subjects. Although the total vitamin B1 level in whole blood was high in the uremic patients, the transketolase activity in the hemolysate and the thiamine pyrophosphate effect on it ...
M, Kuriyama, A, Mizuma, R, Yokomine, A, Igata, Y, Otuji   +4 more
openaire   +2 more sources

Analysis of Blood Transketolase Activity

American Journal of Clinical Pathology, 1971
The determination of blood transketolase (TK) activity is based on the incubation of lysed erythrocytes with and without added thiamine pyrophosphate (TPP). The increased TK activity resulting from the addition of TPP coenzyme is inversely related to thiamine depletion.
M F, Massod, S L, McGuire, K R, Werner
openaire   +2 more sources

Isolation and properties of human transketolase

Biochemistry (Moscow), 2010
Recombinant human (His)(6)-transketolase (hTK) was obtained in preparative amounts by heterologous expression of the gene encoding human transketolase in Escherichia coli cells. The enzyme, isolated in the form of a holoenzyme, was homogeneous by SDS-PAGE; a method for obtaining the apoenzyme was also developed.
L E, Meshalkina, O N, Solovjeva, Yu A, Khodak, V L, Drutsa, G A, Kochetov   +4 more
openaire   +2 more sources

Donor substrate regulation of transketolase

European Journal of Biochemistry, 2004
The influence of substrates on the interaction of apotransketolase with thiamin diphosphate was investigated in the presence of magnesium ions. It was shown that the donor substrates, but not the acceptor substrates, enhance the affinity of the coenzyme either to only one active center of transketolase or to both active centers, but to different ...
Olga A, Esakova, Ludmilla E, Meshalkina, Ralph, Golbik, Gerhard, Hübner, German A, Kochetov   +4 more
openaire   +2 more sources

Anaemia and Erythrocyte Transketolase Activity

Acta Haematologica, 2009
Erythrocyte transketolase activity is raised in megaloblastic anaemia due to vitamin B₁₂ deficiency and inversely related to haemoglobin concentration. It si usually normal in cases of megaloblastic anaemia due to folate deficiency though sufficient exceptions occur to invalidate the use of red cell transketolase assay in ...
D G, Wells, V, Marks
openaire   +2 more sources