Results 131 to 140 of about 14,370 (182)
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Tubulin diversity in trophozoites of Giardia lamblia
Histochemistry and Cell Biology, 2003Giardia lamblia is a diplomonad that parasitizes the small intestine of vertebrates. The trophozoite is multiflagellar and its cytoskeleton presents a complex organization of microtubular structures. One of these, the adhesive disk, consists of a microtubular spiral.
Loraine, Campanati +5 more
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Cryopreservation of viable Giardia intestinalis trophozoites
Transactions of the Royal Society of Tropical Medicine and Hygiene, 1984A technique is described for the cryopreservation of Giardia intestinalis trophozoites. The most satisfactory results were obtained when organisms were preserved with either 7.5 or 10% dimethyl sulphoxide (Me2SO) and cooled using a liquid nitrogen controlled freezer. Under these conditions more than 70% of organisms were motile after thawing.
Phillips, R. E. +2 more
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Transcriptional profiling of Entamoeba histolytica trophozoites
International Journal for Parasitology, 2005We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS) genes. The arrays accurately detected message abundance and 31-47% of amebic genes were expressed under standard tissue culture conditions (levels detectable by Northern blot analysis or RT-PCR ...
Ryan C, MacFarlane +2 more
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Pyrimidine metabolism in Giardia lamblia trophozoites
Molecular and Biochemical Parasitology, 1982The pyrimidine metabolism of Giardia lamblia trophozoites (Portland I strain) was studied using whole trophozoites and trophozoite homogenates. Pyrimidines and pyrimidine nucleosides were readily incorporated into nucleic acids. Orotic and aspartic acid incorporations were below the level of detection.
D G, Lindmark, E L, Jarroll
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INHIBITION OF IN VITRO ATTACHMENT OF GIARDIA TROPHOZOITES BY MUCIN
Journal of Parasitology, 2002An enzyme-linked immunoabsorbent assay was developed to quantify the number of Giardia sp. trophozoites attached to a substratum. Trophozoites were allowed to attach for 5 or 10 min to untreated or modified substratum, or allowed to attach in the presence of cytoskeletal inhibitors.
Heidi, Roskens, Stanley L, Erlandsen
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Peroxidase Localization in Hartmanella Trophozoites
Proceedings, annual meeting, Electron Microscopy Society of America, 1971Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa ...
George E. Childs, Joseph H. Miller
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The American Journal of Tropical Medicine and Hygiene, 1935
The purpose of this article is to describe a case in which there is presumptive evidence of self-inoculation with Endamoeba histolytica through contaminated vaseline, and to report an experiment bearing on the longevity of E. histolytica in vaseline at room temperature. Mr. S. P.
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The purpose of this article is to describe a case in which there is presumptive evidence of self-inoculation with Endamoeba histolytica through contaminated vaseline, and to report an experiment bearing on the longevity of E. histolytica in vaseline at room temperature. Mr. S. P.
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The Uptake and Metabolism of Cysteine by Giardia lamblia Trophozoites
Journal of Eukaryotic Microbiology, 1994ABSTRACT. The cysteine, cystine, methionine and sulfate uptake and cysteine metabolism of Giardia lamblia was studied. Initial experiments indicated that bathocuproine sulphonate (20 μM) added to Keister's modified TYI‐S‐33 medium supported the growth of G. lamblia at low L‐cysteine concentration.
H D, Lujan, T E, Nash
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Oral Infection of Mice with Trophozoites of Giardia muris
The American Journal of Tropical Medicine and Hygiene, 1977Giardia muris has been maintained for years in the mouse by administering by gavage 10,000 trophozoites taken from the small intestine of infected mice. Despite the growth of numerous protozoa in the intestine, absorption of tetracycline hydrochloride in infected mice was not changed to a statistically significant extent, as blood drug levels increased
I, de Carneri, F, Trane
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Menadione kills trophozoites and cysts of Giardia intestinalis
Microbiology, 2004Production of reactive oxygen species by redox cycling in the presence of low levels of oxygen has been studied as a possible approach to anti-protozoal chemotherapeutic strategy. Incubation of the diplomonad flagellateGiardia intestinaliswith 2-methy-1,4-naphthoquinone (menadione), under anaerobic conditions, gave UV absorption changes characteristic ...
Timothy, Paget +5 more
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