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Inactivation of immobilized trypsin under dissimilar conditions produces trypsin molecules with different structures

, 2016
Bovine trypsin has been immobilized on glyoxyl-agarose and two different preparations have been produced. One was reduced just after immobilization, while the other was left to continue the enzyme-support reaction.
Alfredo Sánchez   +7 more
semanticscholar   +1 more source

A Supramolecular Vesicle Based on the Complexation of p-Sulfonatocalixarene with Protamine and its Trypsin-Triggered Controllable-Release Properties.

Chemistry, 2016
Enzyme-responsive assembly represents one of the increasingly significant topics in biomaterials research and finds feasible applications to the controlled release of therapeutic agents at specific sites at which the target enzymes are located.
Kui Wang   +3 more
semanticscholar   +1 more source

A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.

Canadian Journal of Biochemistry and Physiology, 1959
The spectrophotometric procedure proposed by Schwert and Takenaka for the assay of chymotrypsin and trypsin has been modified and extended to include the application to N-benzoyl-L-tyrosine ethyl ester and α-p-toluenesulphonyl-L-arginine methyl ester ...
B. Hummel
semanticscholar   +1 more source

Potential toxicity of phthalic acid esters plasticizer: interaction of dimethyl phthalate with trypsin in vitro.

Journal of Agricultural and Food Chemistry, 2015
Dimethyl phthalate (DMP) is widely used as a plasticizer in industrial processes and has been reported to possess potential toxicity to the human body. In this study, the interaction between DMP and trypsin in vitro was investigated.
Yaping Wang, Guowen Zhang, Langhong Wang
semanticscholar   +1 more source

Heat of reaction between trypsin and soybean trypsin inhibitor

Archives of Biochemistry and Biophysics, 1952
Abstract Calorimetric determinations of the heat of reaction between trypsin and soybean trypsin inhibitor protein indicate that ΔH for the reaction at 25 ° is 0 ± 1 kilocal./mole of trypsin reacted, if similar experiments with the inhibitor protein and bovine serum albumin are accepted as valid controls.
Julian M. Sturtevant, Alan Dobry
openaire   +3 more sources

Nostosins, Trypsin Inhibitors Isolated from the Terrestrial Cyanobacterium Nostoc sp. Strain FSN.

Journal of Natural Products, 2014
Two new trypsin inhibitors, nostosin A (1) and B (2), were isolated from a hydrophilic extract of Nostoc sp. strain FSN, which was collected from a paddy field in the Golestan Province of Iran.
Liwei Liu   +8 more
semanticscholar   +1 more source

A radioactive method for the measurement of trypsin and trypsin-like activities

Analytical Biochemistry, 1989
A simple and highly sensitive method for the assay of trypsin has been developed by making use of the phosphorylated synthetic peptide Leu-Arg-Arg-Ala-Ser-(32P)-Leu-Gly as substrate. The technique has been adapted from the phosphocellulose method of R. Roskoski, Jr. (in Methods in Enzymology (Corbin, J., and Hardman, J., Eds.), Vol. 99, pp.
Susana Passeron   +3 more
openaire   +3 more sources

Effects of bisphenol S on the structures and activities of trypsin and pepsin.

Journal of Agricultural and Food Chemistry, 2014
The effects of bisphenol S on the structures and activities of trypsin and pepsin were investigated by various methods like UV-visible absorbance, fluorescence, circular dichroism, and molecular docking.
Yanqing Wang, Hong-Mei Zhang
semanticscholar   +1 more source

Large-scale quantitative assessment of different in-solution protein digestion protocols reveals superior cleavage efficiency of tandem Lys-C/trypsin proteolysis over trypsin digestion.

Journal of Proteome Research, 2012
The complete and specific proteolytic cleavage of protein samples into peptides is crucial for the success of every shotgun LC-MS/MS experiment. In particular, popular peptide-based label-free and targeted mass spectrometry approaches rely on efficient ...
Timo Glatter   +5 more
semanticscholar   +1 more source

Interaction between four flavonoids and trypsin: effect on the characteristics of trypsin and antioxidant activity of flavonoids

, 2014
Summary Interaction of flavonoids and enzyme may affect characteristics and physiological activities of both components. In this study, the effects of the interaction between four flavonoids (quercetin, luteolin, kaempferol and apigenin) and trypsin ...
Qiong Li   +4 more
semanticscholar   +1 more source

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