Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Twin Challenges in Türkiye: Exclusive Breastfeeding Rates and Predictors of Breastfeeding Duration in a Tertiary Care Center. [PDF]
Kömürlüoğlu A, Çıplak G.
europepmc +1 more source
Genetic diversity and population structure of the Taigan dog breed
The Taigan is a rare sighthound from the mountains of Kyrgyzstan. We used DNA markers to study its genetic diversity and compare it with other sighthound breeds. Our findings show that the Taigan shares close genetic ties with the Kazakh Tazy, suggesting a common ancestry shaped by nomadic traditions and regional adaptation.
Kira Bespalova+10 more
wiley +1 more source
Genetic and Environmental Effects on Parent-Rated Adaptive Behaviour in Infancy. [PDF]
Halkola H+5 more
europepmc +1 more source
Digital twin manifesto for the pathology laboratory. [PDF]
Eccher A+9 more
europepmc +1 more source
Digital twins, synthetic patient data, and in-silico trials: can they empower paediatric clinical trials? [PDF]
Pammi M+5 more
europepmc +1 more source
Genetic Factors Explain Half of the Individual Susceptibility to Chronic Bronchitis, Airflow Obstruction and COPD Regardless of the Spirometric Definition: A Nationwide Twin Study. [PDF]
Meteran H+6 more
europepmc +1 more source
Enhancing clinical outcome predictions through effective sample size evaluation in graph-based digital twin modeling. [PDF]
Li X+4 more
europepmc +1 more source
Source-tracking Klebsiella outbreaks in premature infants using a novel amplicon fingerprinting method. [PDF]
Matson AP+7 more
europepmc +1 more source