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Ultracentrifugation-Free Enrichment and Quantification of Small Extracellular Vesicles.
Analytical Chemistry, 2022Cancer is a malignant tumor with the highest mortality of human diseases. The early diagnosis of cancer can greatly reduce its mortality. Ultracentrifugation is the most commonly employed technique to separate small extracellular vesicles (sEVs) due to ...
Xingle Yu +13 more
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Journal of Proteome Research, 2020
Exosomes, a subtype of extracellular vesicles secreted by mammalian cells with a typical size range of 30-150 nm, have been implicated in many biological processes as intercellular communication carriers.
Sheng Guan +5 more
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Exosomes, a subtype of extracellular vesicles secreted by mammalian cells with a typical size range of 30-150 nm, have been implicated in many biological processes as intercellular communication carriers.
Sheng Guan +5 more
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Required ultracentrifugal time for condensing solution by ultracentrifuge
Bioprocess Engineering, 1990Required ultracentrifugal time for condensing a solution by ultracentrifuge with an angle rotor or a swing rotor was estimated by considering the calculated distribution of concentration in an ultracentrifugal tube. From the calculated distribution of the concentration a free boundary between solvent and solution was found in the ultracentrifugal tube ...
T. Kajiuchi, K. Ohtomo, N. Shiragami
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Ultracentrifugation of Salivary Mucins [PDF]
, 1982 The salivary mucins contribute to the protection of enamel against the attack by bacteria and their metabolic products among others by the formation of a protein pellicle on its surface. To study the interaction of salivary mucins with enamel and bacteria, pure and well characterized mucins should be available. A widely used method for the isolation of
A.V. Nieuw Amerongen +2 more
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A vacuum ultracentrifuge [PDF]
Journal of Physics E: Scientific Instruments, 1975 An ultracentrifuge used to magnetically support and spin small rotors to high rotational speeds is described. Electronic circuits for the magnetic support system and the rotor drive are given. The highest speed attained with the equipment was 17500 rev s-1.
M S Brown, G F R C Morris
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Lipid Exchange by Ultracentrifugation
2016Lipids play an important role in maintaining P-type ATPase structure and function, and often they are crucial for ATPase activity. When the P-type ATPases are in the membrane, they are surrounded by a mix of different lipid species with varying aliphatic chain lengths and saturation, and the complex interplay between the lipids and the P-type ATPases ...
Nikolaj D. Drachmann +2 more
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Ultracentrifugation of Acetylated Thrombin
Thrombosis and Haemostasis, 1960SummaryAcetylated bovine thrombin with a specific activity of 5930 units per milligram dry weight was found to be homogeneous by ultracentrifuge analysis. The sedimentation constant in units of 10−13 sec. at 20° C, in 0.1 M potassium chloride was 3.3. This constant is not concentration dependent.
Robert S. Shepard +2 more
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Coping with convection in the ultracentrifuge
Analytical Biochemistry, 1978Abstract If cell orientation and temperature control are good, convective flow in the analytical ultracentrifuge cell occurs due to vibrations transmitted from a faulty drive to the rotor itself. Minor vibrations are usually “ironed out” at high speeds but, at low speeds, (
David B. Millar, John P. Christopher
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Calibrating analytical ultracentrifuges
European Biophysics Journal, 2021Analytical ultracentrifugation (AUC) is based on the concept of recording and analyzing macroscopic macromolecular redistribution that results from a centrifugal force acting on the mass of suspended macromolecules in solution. Since AUC rests on first principles, it can provide an absolute measurement of macromolecular mass, sedimentation and ...
Huaying Zhao +3 more
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Analytical Ultracentrifugation
Current Protocols in Protein Science, 1996AbstractAnalytical ultracentrifugation is one of the most powerful, though as yet underexploited, techniques available to molecular biology and biochemistry. This overview describes applications for analytical ultracentrifugation along with important considerations relating to experimental design.
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