Results 101 to 110 of about 5,155,579 (288)

Evolutionary divergence and functional insights into the heteromeric cis‐prenyltransferase of Paramecium tetraurelia

open access: yesThe FEBS Journal, EarlyView.
Heteromeric cis‐prenyltransferases (CPT) are indispensable for dolichol synthesis and protein N‐glycosylation in most eukaryotes. The catalytic subunits are strongly conserved throughout evolution, in contrast to the evolutionarily variable accessory subunits. The POC1 protein from Paramecium tetraurelia is the smallest identified CPT‐accessory subunit
Agnieszka Onysk   +8 more
wiley   +1 more source

Leishmania donovani's protein tyrosine phosphatases interact with DUF21 and respond to environmental magnesium

open access: yesThe FEBS Journal, EarlyView.
The Leishmania phosphatase PTP1, and possibly the genetically similar PTP2, interacts with the Leishmania transmembrane protein DUF21. When both ptp1 and ptp2 are knocked out of Leishmania (LdΔPTP1/2), the parasite can no longer survive without magnesium in vitro and has reduced viability in the host macrophage. Conversely, in duf21 knockout (LdΔDUF21),
Kayla Paulini   +6 more
wiley   +1 more source

Scrutinizing UML Teaching and Learning Modeling Tools

open access: diamond, 2023
Abdulqader Almasabe   +2 more
openalex   +1 more source

Sensing of cardiolipin exposure on plasma membranes of apoptotic cells by EryA‐mCherry protein

open access: yesThe FEBS Journal, EarlyView.
An aegerolysin protein erylysin A (EryA) fused with mCherry binds to artificial lipid vesicles supplemented with cardiolipin (CL). This binding is much more specific than that of annexin V, which binds various negatively charged glycerophospholipids (PG, PS, PI). In mammalian cells, the CL exposition at the membrane surface represents an early hallmark
Luka Žeželj   +12 more
wiley   +1 more source

Modeling and Analysis of a LOX/Ethanol Liquid Rocket Engine

open access: yesJournal of Aerospace Technology and Management, 2018
This work studies the performance and dry mass of the under development LOX/Ethanol L75 liquid rocket engine. To this end, an object-oriented program written in C++ was developed.
Fábio Antônio da Silva Mota   +3 more
doaj  

Oxygen‐independent expression of HIF‐1α during the cell cycle in hepatocellular carcinoma cells controls essential metabolic pathways under normoxia

open access: yesThe FEBS Journal, EarlyView.
In Huh7 cells, HIF‐1α is essential as it maintains the expression of proteins involved in glycolysis and steroid/cholesterol biosynthesis both under normoxia and hypoxia. On the other hand, in HeLa cells, these pathways are induced by HIF‐1α only under hypoxia.
Ioanna‐Maria Gkotinakou   +6 more
wiley   +1 more source

Cellular Morphometric Analysis (CellMorph)—a comprehensive imaging‐based tool for quantifying cellular phenotype heterogeneity and dynamics across biological processes

open access: yesThe FEBS Journal, EarlyView.
CellMorph is a groundbreaking tool that allows researchers to analyze multiple features from single cells, including cell size and shape, cytosolic staining and subcellular texture, nuclear staining, and morphometry. By evaluating images from both bright‐field and fluorescence microscopy, it also tracks changes in living cells during key processes such
Henrique Quaiato de Oliveira   +16 more
wiley   +1 more source

EHD4 and ASAP2 are critical negative regulators of the claudin‐5‐based endothelial barrier

open access: yesThe FEBS Journal, EarlyView.
Cell‐surface CLDN‐5 protein levels can be evaluated using a probe that can bind to the extracellular domains of CLDN‐5. A probe derived from Clostridium perfringens enterotoxin allows us to isolate cells with high CLDN‐5 protein levels from a knockout cell library.
Yosuke Hashimoto   +8 more
wiley   +1 more source

Regulation of PHOX2B gene expression by the long non‐coding natural antisense RNA PHOX2B‐AS1

open access: yesThe FEBS Journal, EarlyView.
PHOX2B is a transcription factor essential for autonomic nervous system development. We identify and characterize PHOX2B‐AS1, a human long non‐coding antisense transcript at the PHOX2B locus, along with its murine counterpart. Our findings reveal bidirectional transcription and reciprocal regulation: PHOX2B activates PHOX2B‐AS1, whereas PHOX2B‐AS1 ...
Simona Di Lascio   +12 more
wiley   +1 more source

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