Nonselective URA3 Colony-Color Assay in Yeast ade1 or ade2 Mutants
BioTechniques, 1997 Yi-shin Weng, Jac A. Nickoloff
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Effects of perinuclear chromosome tethers in the telomeric URA3/5FOA system reflect changes to gene silencing and not nucleotide metabolism [PDF]
Frontiers in Genetics, 2012 Telomeres are repetitive DNA sequences that protect the ends of linear chromosomes. Telomeres also recruit histone deacetylase complexes that can then spread along chromosome arms and repress the expression of subtelomeric genes in a process known as ...
Betty Po Kei Poon +2 more
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Characterizing the Fitness Effects of Mutations in the Yeast URA3 Gene [PDF]
Biophysical Journal, 2009 The central goal of this project is to study the mechanisms by which changes in gene sequence affect the level of function of the associated protein and therefore, fitness of the organism that carries a particular allele of a gene. In order to study this process, we performed random mutagenesis of the yeast URA3 gene and are currently assaying the ...
Anupriya Singhal
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Acetate‐Mediated Production of Orotic Acid by ura3 Mutants of Candida albicans [PDF]
Microbiology and Immunology, 2004 AbstractCandida albicans ura3 mutants were found to produce large amounts of orotic acid when the growth medium was supplemented with sodium acetate. Experiments with 13C‐labeled acetate showed that the acetate served as a precursor of orotic acid.
Takashi Nezu, Osamu Shimokawa
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Oxalurate Induction of Multiple URA3 Transcripts in Saccharomyces cerevisiae [PDF]
Molecular and Cellular Biology, 1983 The URA3 gene from Saccharomyces cerevisiae is localized on a 1.1-kilobase (kb) DNA fragment. By using this fragment as a hybridization probe, we found that oxalurate, a gratuitous inducer of the allantoin degradative system, also serves to induce URA3 specific RNA. This response is restricted to oxalurate; other conditions which bring about high-level
Richard G. Buckholz, Terrance Cooper
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SIR2 Expression Noise Can Generate Heterogeneity in Viability but Does Not Affect Cell-to-Cell Epigenetic Silencing of Subtelomeric URA3 in Yeast [PDF]
G3: Genes, Genomes, Genetics, 2020 Chromatin structure clearly modulates gene expression noise, but the reverse influence has never been investigated, namely how the cell-to-cell expression heterogeneity of chromatin modifiers may generate variable rates of epigenetic modification.
Jian Liu +4 more
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The development and application of a multiple gene co-silencing system using endogenous URA3 as a reporter gene in Ganoderma lucidum. [PDF]
PLoS ONE, 2012 Ganoderma lucidum is one of the most important medicinal mushrooms; however, molecular genetics research on this species has been limited due to a lack of reliable reverse genetic tools.
Dashuai Mu +6 more
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cis- and trans-acting regulatory elements of the yeast URA3 promoter. [PDF]
Molecular and Cellular Biology, 1990 Expression of the yeast pyrimidine biosynthetic gene, URA3, is induced three- to fivefold in response to uracil starvation, and this regulation is mediated by the transcriptional activator PPR1 (pyrimidine pathway regulator 1). In this study, we have analyzed the regulatory elements of the URA3 promoter by DNase I footprinting, using partially purified
A. K. Roy, F. Exinger, Régine Losson
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Efficient multiple gene knock-out in Colletotrichum higginsianum via CRISPR-Cas9 ribonucleoprotein and URA3 -based marker recycling [PDF]
, 2023 AbstractColletotrichum higginsianum is a hemibiotrophic pathogen that causes anthracnose disease on crucifer hosts, including Arabidopsis thaliana. Despite the availability of genomic and transcriptomic information and the ability to transform both organisms, identifying C.
Katsuma Yonehara +6 more
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Predicting the Evolution of Gene $ura3$ in the Yeast Saccharomyces Cerevisiae [PDF]
, 2017 Published in Procedia Computer Science.
Jacques M. Bahi +2 more
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