Results 161 to 170 of about 32,450 (192)

Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans

Microbiology, 2005
HWP1 encodes an adhesin of Candida albicans and has been implicated in filamentation and virulence. URA3, an often-used transformation selection marker, is apparently incorrectly expressed when integrated at the HWP1 locus, which results in an attenuated virulence phenotype. Expression of URA3 is compromised by ectopic integration at other loci as well.
Laura L. Sharkey, Wei-li Liao, Anup Ghosh, William A. Fonzi   +3 more
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Yeast promoters URA1 and URA3

Journal of Molecular Biology, 1985
Transcription of the two unlinked structural genes URA1 and URA3 of Saccharomyces cerevisiae is positively regulated by the gene product PPR1. We have used S1 digestion and primer extension mapping to investigate the RNAs produced in different genetic backgrounds: wild-type, ppr1 deletion mutants, constitutively induced and non-inducible ppr1 mutants ...
R. Losson, R.P.P. Fuchs, F. Lacroute
openaire   +2 more sources

Yeast artificial chromosome fragmentation vectors that utilize URA3 selection

Genetic Analysis: Biomolecular Engineering, 1993
Two fragmentation vectors, pSE1 and pSE2, were developed for targeting yeast artificial chromosomes (YACs) containing human genomic DNA. Ura- yeast cells containing YACs were selected with 5-fluoro-orotic acid. Fragmented YACs were subsequently generated by transformation to a Ura+ phenotype.
J R, Cook, S L, Emanuel, S, Pestka
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A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant

Gene, 1982
Dictyostelium discoideum DNA fragments have been inserted into the chimeric bacterium-yeast plasmid YEp13. Recombinant plasmids were used to transform yeast using a strain of Saccharomyces cerevisiae deficient in OMP decarboxylase activity. Several clones were selected for growth in uracil-free medium.
E, Boy-Marcotte, M, Jacquet
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A recyclableCandida albicans URA3 cassette for PCR product-directed gene disruptions

Yeast, 2000
For some time, gene disruptions in Candida albicans have been made with the hisG-URA3-hisG ('Ura-blaster') cassette, which can be re-used in successive transformations of a single strain after homologous excision of URA3. However, the hisG repeats are too large for efficient PCR amplification of the entire cassette, so it cannot be used for PCR product-
R B, Wilson, D, Davis, B M, Enloe, A P, Mitchell   +3 more
openaire   +2 more sources

Cloning of the C-URA3 gene and construction of a triple auxotroph (his5, ade1, ura3) as a useful host for the genetic engineering of Candida maltosa

Current Genetics, 1993
The C-URA3 gene of the n-alkane assimilating-yeast Candida maltosa was cloned by complementation of the ura3 mutation of Saccharomyces cerevisiae. The nucleotide sequence of C-URA3 and its deduced amino-acid sequence showed significant homology to those of the orotidine 5'-phosphate decarboxylases of other fungal species. To construct a useful host for
M, Ohkuma, S, Muraoka, C W, Hwang, A, Ohta, M, Takagi   +4 more
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Use of URA3 as a reporter of gene expression in C. albicans

Current Genetics, 1995
The C. albicans URA3 gene was tested as a reporter of gene expression. An integrating vector was constructed which contained ADE2 as a selectable marker together with a truncated form of URA3 lacking the first three codons. A DNA fragment containing the promoter and the first 90 codons of the C. albicans CEF3 gene was inserted into the unique XhoI site
K K, Myers, P S, Sypherd, W A, Fonzi
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Structure and function of the yeast URA3 gene: expression in Escherichia coli

Gene, 1984
The expression of the cloned Saccharomyces cerevisiae URA3 gene in Escherichia coli on both plasmid and phage vectors was studied. Isolates of the gene from two different laboratory strains of yeast differ in their ability to be expressed in E. coli in the absence of external adjacent promoters of transcription.
M, Rose, P, Grisafi, D, Botstein
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Isolation and characterization of Candida kefyr orotidine‐5′‐phosphate decarboxylase (URA3) gene

Yeast, 2009
AbstractCandida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine‐5′‐phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C.
Chu, JKM, Tsang, PWK, Wong, KS
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Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana

Applied Microbiology and Biotechnology, 2012
Genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as Beauveria bassiana. To date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduction of genes into the fungus.
Sheng-Hua, Ying, Ming-Guang, Feng, Nemat O, Keyhani   +2 more
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