Modified protocol for URA3 counter-selection at highly expressed regions of Saccharomyces cerevisiae v1 [PDF]
, 2020 This is a modified protocol for URA3 counter-selection in S. cerevisiae and similar yeasts. It is identical to the standard counter-selection with two transformations, with the exception of the replica-plating step. This protocol is appropriate for genomic loci where URA3 is over-expressed and the Ura3 protein is likely to be present in the colonies ...
Lenny Teytelman, Jasper Rine
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Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker [PDF]
Molecular Microbiology, 2000 To facilitate the functional genomic analysis of an archaeon, we have developed a homologous gene replacement strategy for Halobacterium salinarum based on ura3, which encodes the pyrimidine biosynthetic enzyme orotidine‐5′‐monophosphate decarboxylase. H. salinarum was shown to be sensitive to 5‐fluoroorotic acid (5‐FOA), which can select for mutations
Ronald F. Peck +2 more
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Expanding tunable selection in yeast using auxotrophic markers URA3 and TRP1 [PDF]
Abstract Auxotrophic markers are fundamental tools in yeast genetics, but their use is traditionally binary (growth or no growth), which limits quantitative analysis. Here we repurpose the classic auxotrophic markers URA3 and TRP1 as tunable selection tools that quantitatively link gene-expression levels to growth outcomes.
Youqing Luo, Tatsuhisa Tsuboi
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Nucleotide sequences of theTrichoderma reeseiura3 (OMPdecase) and ura5 (OPRTase) genes
Nucleic Acids Research, 1990 Thierry Bergès +2 more
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Direct selection of stabilised yeastURA3transformants with 5-fluorouracil
Nucleic Acids Research, 1991 Michael A. Romanos +2 more
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Neocentromeres form efficiently at multiple possible loci in Candida albicans. [PDF]
PLoS Genetics, 2009 Centromeres are critically important for chromosome stability and integrity. Most eukaryotes have regional centromeres that include long tracts of repetitive DNA packaged into pericentric heterochromatin.
Carrie Ketel +7 more
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Removing auto-activators from yeast-two-hybrid assays by conditional negative selection
Scientific Reports, 2021 Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein–protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing ...
Devendra Shivhare +4 more
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Improving the efficiency of homologous recombination by chemical and biological approaches in Yarrowia lipolytica. [PDF]
PLoS ONE, 2018 Gene targeting is a challenge in Yarrowia lipolytica (Y. lipolytica) where non-homologous end-joining (NHEJ) is predominant over homologous recombination (HR). To improve the frequency and efficiency of HR in Y.
In-Seung Jang +4 more
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Size of gene specific inverted repeat--dependent gene deletion In Saccharomyces cerevisiae. [PDF]
PLoS ONE, 2013 We describe here an approach for rapidly producing scar-free and precise gene deletions in S. cerevisiae with high efficiency. Preparation of the disruption gene cassette in this approach was simply performed by overlap extension-PCR of an invert repeat ...
Chanyuen Lim +5 more
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Counter-selectable marker for bacterial-based interaction trap systems
BioTechniques, 2006 Counter-selectable markers can be used in two-hybrid systems to search libraries for a protein or compound that interferes with a macromolecular interaction or to identify macromolecules from a population that cannot mediate a particular interaction.
Xiangdong Meng +4 more
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