Results 21 to 30 of about 32,450 (192)

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

BMC Biotechnology, 2020
Background Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only ...
Oliver Blechert, Huan Mei, Xiaohui Zang, Hailin Zheng, Guanzhao Liang, Weida Liu   +5 more
doaj   +1 more source

The IQ-compete assay for measuring mitochondrial protein import efficiencies in living yeast cells. [PDF]

FEBS Lett
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Hoffman Y, Egeler A, Rödl S, Herrmann JM.   +3 more
europepmc   +2 more sources

Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers. [PDF]

FEBS Lett
Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Gaur D, Acquaviva B, Smith BA, Walker N, Walter I, Wohlever ML.   +5 more
europepmc   +2 more sources

Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast

BMC Biotechnology, 2004
Background A valuable weapon in the arsenal available to yeast geneticists is the ability to introduce specific mutations into yeast genome. In particular, methods have been developed to introduce deletions into the yeast genome using PCR fragments ...
Honigberg Saul M, Kupiec Martin, Gray Misa   +2 more
doaj   +1 more source

Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells

Journal of Fungi, 2020
The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis ...
Manuela Gómez-Gaviria, Nancy E. Lozoya-Pérez, Monika Staniszewska, Bernardo Franco, Gustavo A. Niño-Vega, Hector M. Mora-Montes   +5 more
doaj   +1 more source

Candida albicans Cells Lacking AP-2 Have Defective Hyphae and Are Avirulent Despite Increased Host Uptake and Intracellular Proliferation in Macrophages. [PDF]

Mol Microbiol
Mutation of exocytic cargo adapter apm4Δ/Δ results in reduced virulence of Candida albicans during infection despite promoting intracellular growth in macrophages. ABSTRACT Candida albicans is a commensal microbe and opportunistic human pathogen. Candida yeast are recognized and taken up by macrophages via phagocytosis.
Christou S, Evans S, Knafler H, Rooij IS, Ayscough KR, Johnston SA.   +5 more
europepmc   +2 more sources

PCR- and Ligation-Mediated Synthesis of Split-Marker Cassettes with Long Flanking Homology Regions for Gene Disruption in Candida albicans

BioTechniques, 2000
Because Candida albicans is a diploid organism, two consecutive steps of gene disruption are required to generate a gene knock-out. The same marker (URA3) is often used for disruption of both copies of the gene.
Ronald de Hoogt, Walter H.M.L. Luyten, Roland Contreras, Marianne D. De Backer   +3 more
doaj   +1 more source

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Microbial Biotechnology, 2020
Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species.
Ke Liu, Bin Sun, Hao You, Jun‐Liang Tu, Xuya Yu, Peng Zhao, Jun‐Wei Xu   +6 more
doaj   +1 more source

Cryptococcus neoformans Prp8 Intein: An In Vivo Target-Based Drug Screening System in Saccharomyces cerevisiae to Identify Protein Splicing Inhibitors and Explore Its Dynamics

Journal of Fungi, 2022
Inteins are genetic mobile elements that are inserted within protein-coding genes, which are usually housekeeping genes. They are transcribed and translated along with the host gene, then catalyze their own splicing out of the host protein, which assumes
José Alex Lourenço Fernandes, Matheus da Silva Zatti, Thales Domingos Arantes, Maria Fernanda Bezerra de Souza, Mariana Marchi Santoni, Danuza Rossi, Cleslei Fernando Zanelli, Xiang-Qin Liu, Eduardo Bagagli, Raquel Cordeiro Theodoro   +9 more
doaj   +1 more source

Efficient construction of homozygous diploid strains identifies genes required for the hyper-filamentous phenotype in Saccharomyces cerevisiae. [PDF]

PLoS ONE, 2011
Yeast cells undergo diploid-specific developments such as spore formation via meiosis and pseudohyphal development under certain nutrient-limited conditions.
Kentaro Furukawa, Takako Furukawa, Stefan Hohmann   +2 more
doaj   +1 more source