Results 31 to 40 of about 32,450 (192)
Recombinogenic conditions influence partner choice in spontaneous mitotic recombination. [PDF]
PLoS Genetics, 2013 Mammalian common fragile sites are loci of frequent chromosome breakage and putative recombination hotspots. Here, we utilized Replication Slow Zones (RSZs), a budding yeast homolog of the mammalian common fragile sites, to examine recombination ...
James D Cauwood +3 more
doaj +1 more source
G3: Genes, Genomes, Genetics, 2019 Candida albicans, the most common fungal pathogen, is a diploid with a genome that is rich in repeats and has high levels of heterozygosity. To study the role of different recombination pathways on direct-repeat recombination, we replaced either allele ...
Alberto Bellido +5 more
doaj +1 more source
Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates. [PDF]
PLoS ONE, 2016 Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis.
Xin Fan +8 more
doaj +1 more source
CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica
Metabolic Engineering Communications, 2020 CRISPR-Cas9 has been widely adopted as the basic toolkit for precise genome-editing and engineering in various organisms. Alternative to Cas9, Cas12 or Cpf1 uses a simple crRNA as a guide and expands the protospacer adjacent motif (PAM) sequence to TTTN.
Zhiliang Yang, Harley Edwards, Peng Xu
doaj +1 more source
Formation of AAV single stranded DNA genome from a circular plasmid in Saccharomyces cerevisiae.
PLoS ONE, 2011 Adeno-associated virus (AAV)-based vectors are promising tools for targeted transfer in gene therapy studies. Many efforts have been accomplished to improve production and purification methods.
Tiziana Cervelli +2 more
doaj +1 more source
Journal of Fungi, 2023 The CRISPR/Cas9 system has become a popular approach to genome editing. Compared with the plasmid-dependent CRISPR system, the ribonucleoprotein (RNP) complex formed by the in vitro assembly of Cas9 and single-guide RNA (sgRNA) has many advantages ...
Yi Tan +7 more
doaj +1 more source
Cis-unsaturated sphingolipids support growth of sphingoid base-deficient yeast but impair plasma membrane integrity. [PDF]
FEBS Open BioSphingoid base structures, the sphingolipid backbones, vary among species. We established yeast cells in which the native sphingoid base was replaced with plant‐type bases containing cis or trans double bonds. This is, to our knowledge, the first eukaryotic model mostly composed of sphingolipids containing cis‐unsaturated sphingoid base, providing a ...
Higuchi T +5 more
europepmc +2 more sources
A single heterochromatin boundary element imposes position-independent antisilencing activity in Saccharomyces cerevisiae minichromosomes. [PDF]
PLoS ONE, 2011 Chromatin boundary elements serve as cis-acting regulatory DNA signals required to protect genes from the effects of the neighboring heterochromatin. In the yeast genome, boundary elements act by establishing barriers for heterochromatin spreading and ...
Sangita A Chakraborty +2 more
doaj +1 more source
Disruption to de novo uridine biosynthesis alters β-1,3-glucan masking in Candida albicans
mSphereThe uridine derivatives UDP-glucose and UDP-N-acetylglucosamine are important for cell wall construction as they are the precursors for the synthesis of β-1,3-glucan and chitin, respectively.
Mikayla M. Mangrum +8 more
doaj +1 more source
G3: Genes, Genomes, Genetics, 2019 In Saccharomyces cerevisiae, the meiosis-specific axis proteins Hop1 and Red1 are present nonuniformly across the genome. In a previous study, the meiosis-specific VMA1-derived endonuclease (VDE) was used to examine Spo11-independent recombination in a ...
Anura Shodhan +2 more
doaj +1 more source