Results 41 to 50 of about 32,450 (192)
BMC Genomics, 2003 Background Transformation-associated recombination (TAR) cloning in yeast is a unique method for selective isolation of large chromosomal fragments or entire genes from complex genomes.
Barrett J Carl +5 more
doaj +1 more source
A Modular and Customizable CRISPR/Cas Toolkit for Epigenome Editing of Cis-regulatory Modules. [PDF]
Adv Sci (Weinh)Epigenome editing surpasses genome editing in gene regulation. Modular epigenome editing frameworks are developed to interrogate cis‐regulome, enabling tunable reprogramming of cis‐elements, uncovering their epigenetic responsiveness and cross‐species portability.
Zhang L +6 more
europepmc +2 more sources
Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast.
PLoS ONE, 2013 Polypeptone is widely excluded from Schizosaccharomyces pombe growth medium. However, the reasons why polypeptone should be avoided have not been documented.
Yuzy Matsuo +7 more
doaj +1 more source
Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants. [PDF]
Molecular and Cellular Biology, 1987 A method for introducing specific mutations into the diploid Candida albicans by one-step gene disruption and subsequent UV-induced recombination was developed. The cloned C. albicans URA3 gene was disrupted with the C. albicans ADE2 gene, and the linearized DNA was used for transformation of two ade2 mutants, SGY-129 and A81-Pu.
R, Kelly +3 more
openaire +2 more sources
Vector for Pop-In/Pop-Out Gene Replacement in Pichia pastoris
BioTechniques, 2001 Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of ...
Jon Soderholm +2 more
doaj +1 more source
Frontiers in Microbiology, 2021 Yarrowia lipolytica is known to be capable of metabolizing glucose and accumulating lipids intracellularly; however, it lacks the cellulolytic enzymes needed to break down cellulosic biomass directly. To develop Y.
Hui Wei +8 more
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The FEBS Journal, EarlyView.Heteromeric cis‐prenyltransferases (CPT) are indispensable for dolichol synthesis and protein N‐glycosylation in most eukaryotes. The catalytic subunits are strongly conserved throughout evolution, in contrast to the evolutionarily variable accessory subunits. The POC1 protein from Paramecium tetraurelia is the smallest identified CPT‐accessory subunit
Agnieszka Onysk +8 more
wiley +1 more source
Microorganisms, 2021 Metschnikowia pulcherrima is a non-conventional yeast with the potential to be used in biotechnological processes, especially involving low-cost feedstock exploitation.
Mauro Moreno-Beltrán +3 more
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The FEBS Journal, EarlyView.Lipases are vital in modifying lipid substrates across industries such as food, cosmetics, and pharmaceuticals. Among their features, fatty acid selectivity is particularly important for industrial applications. Rhizopus oryzae lipase (ROL) stands out for its high selectivity and broad applicability.
Zehui Dong +3 more
wiley +1 more source