Results 71 to 80 of about 32,450 (192)
Frontiers in Microbiology, 2018 Saccharomyces boulardii, a subspecies of Saccharomyces cerevisiae, is a well-known eukaryotic probiotic with many benefits for human health. In the present study, a recombinant strain of S.
Ghasem Bagherpour +7 more
doaj +1 more source
Microhomology-mediated end joining induces hypermutagenesis at breakpoint junctions. [PDF]
PLoS Genetics, 2017 Microhomology (MH) flanking a DNA double-strand break (DSB) drives chromosomal rearrangements but its role in mutagenesis has not yet been analyzed. Here we determined the mutation frequency of a URA3 reporter gene placed at multiple locations distal to ...
Supriya Sinha +7 more
doaj +1 more source
Dual Phosphorylation of Thr175 and Ser176 Is Essential for SnRK1α1 Activation
Physiologia Plantarum, Volume 178, Issue 1, January/February 2026.ABSTRACT SnRK1 protein kinases play a pivotal role in regulating plant development, growth signaling, and stress responses by managing cellular responses to energy fluctuations. SnRK1 activation was thought to depend mainly on the phosphorylation of threonine at position 175 (Thr175) within the activation loop.
Alejandra Ávila +5 more
wiley +1 more source
Multiplexed CRISPR-Cas9-Based Genome Editing of
mSphere, 2019 Microbial production of biofuels and bioproducts offers a sustainable and economic alternative to petroleum-based fuels and chemicals. The basidiomycete yeast Rhodosporidium toruloides is a promising platform organism for generating bioproducts due to ...
Peter B. Otoupal +5 more
doaj +1 more source
Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
FEBS Letters, Volume 599, Issue 24, Page 3669-3684, December 2025.The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji +3 more
wiley +1 more source
Clonal Population of Flucytosine-Resistant Candida tropicalis from Blood Cultures, Paris, France
Emerging Infectious Diseases, 2008 Candida tropicalis is a diploid ascomycetes yeast responsible for 4%–24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C.
Marie Desnos-Ollivier +8 more
doaj +1 more source
Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
Bio-Protocol, 2019 Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial step,
Laura Knighton +3 more
doaj +1 more source
BioTechniques, 2004 We describe a novel assay format for the Gal4-based yeast two-hybrid-system, in which the readout from three different reporter genes is measured sequentially in a single microplate.
David R.H. Evans +3 more
doaj +1 more source
CRISPR‐DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution
Advanced Science, Volume 12, Issue 45, December 4, 2025.This study develops an in vivo targeted mutagenesis system based on CRISPR‐guided bacteriophage T5 or T7 DNA polymerases. This system can enable all possible types of substitution mutations within an expanded mutagenesis window in Escherichia coli and exhibit application potential in different microorganisms, thus accelerating the evolution of ...
Shuaili Chen +8 more
wiley +1 more source
Vectors for Expressing T7 Epitope- and His6 Affinity-Tagged Fusion Proteins in S. cerevisiae
BioTechniques, 1998 We have constructed a series of vectors (YGALSETs) for the expression of epitopeand affinity-tagged fusion proteins in yeast cells using the regulated GAL10 promoter.
Shinichiro Enomoto +2 more
doaj +1 more source