Results 81 to 90 of about 32,450 (192)

Integrative modules for efficient genome engineering in yeast

Microbial Cell, 2017
We present a set of vectors containing integrative modules for efficient genome integration into the commonly used selection marker loci of the yeast Saccharomyces cerevisiae.
Triana Amen, Daniel Kaganovich
doaj   +1 more source

Genetic Selection Based on a Ste6*C-HA-Ura3 Substrate Identifies New Cytosolic Quality Control Alleles in Saccharomyces cerevisiae [PDF]

, 2020
Shu Ning Chan, Rupali Prasad, Paul Matsudaira   +2 more
openalex   +1 more source

Penghilangan gen Lacz dari plasmid pUKC 815 untuk pembentuk vektor ekspresi di ragi (Saccharomyces cerevisiae)

Journal of Biological Researches, 2000
The pUKC 815 plasmid was a shuttle vector. This plasmid had the genetic marker to the urasil (URA3) in yeast and ampicillin resistanced marked in E. coli. The pUKC 815 plasmid also had a promotor gene PGK.
Ahmad Thontowi, Ni Nyoman T P, Mariatun Loegito   +2 more
doaj   +1 more source

System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae

BioTechniques, 2006
Integrative, centromeric, and episomal plasmids are essential for easy, fast, and reliable genetic manipulation of yeast. We constructed a system of shuttle vectors based on the widely used plasmids of the pRS series.
Christof Taxis, Michael Knop
doaj   +1 more source

Full-length Genome of a Ogataea polymorpha strain CBS4732 ura3 Δ reveals large duplicated segments in subtelomeric regions [PDF]

, 2021
Chang Jia, Jinlong Bei, Qi Shao, Hemu Wang, Huan Fan, Tung On Yau, Wenjun Bu, Jishou Ruan, Dongsheng Wei, Shan Gao   +9 more
openalex   +1 more source

Ectopic expression of URA3 can influence the virulence phenotypes and proteome of Candida albicans but can be overcome by targeted reintegration of URA3 at the RPS10 locus.

Eukaryotic cell, 2005
Uridine auxotrophy, based on disruption of both URA3 alleles in diploid Candida albicans strain SC5314, has been widely used to select gene deletion mutants created in this fungus by "Ura-blasting" and PCR-mediated disruption. We compared wild-type URA3 expression with levels in mutant strains where URA3 was positioned either within deleted genes or at
Alexandra, Brand, Donna M, MacCallum, Alistair J P, Brown, Neil A R, Gow, Frank C, Odds   +4 more
openaire   +1 more source

Mutagenic specificity of the food mutagen 2-amino-3-methylimidazo[4,5- f]quinoline in Escherichia coli using the yeast URA3 gene as a target [PDF]

, 1998
Thomas H. Broschard
openalex   +1 more source

Sequence of theKluyveromyces lactisURA3 gene

Nucleic Acids Research, 1987
J R, Shuster, D, Moyer, B, Irvine
openaire   +3 more sources

Ty retrotransposon element based multiple integration toolkit for Saccharomyces cerevisiae

Synthetic and Systems Biotechnology
Extra-high-level overexpression of single or multiple specific proteins by integrating specific genes in the genome is vital to achieve the stable and efficient production of target proteins and metabolites in S. cerevisiae.
Song Gao, Weizhu Zeng, Dong Li, Sha Xu, Jingwen Zhou   +4 more
doaj   +1 more source

High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2 [PDF]

, 1994
Vina W. Yang, Jennifer A. Marks, Brian Davis, Thomas W. Jeffries   +3 more
openalex   +1 more source