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Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilis.
Biochemistry, 1977A uracil specific DNA N-glycosidase activity has been partially purified from crude extracts of Bacillus subtilis. The enzyme has a molecular weight of approximately 24 000 with no subunit structure. It has no requirement for any known cofactors but is inhibited in the presence of Co2+, Fe2+, or Zn2+.
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