Results 141 to 150 of about 5,628,361 (283)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

A Demarcation Full of Tension

open access: yesNordic Journal of Studies in Policing
This article, partly based on declassified police and military documents, investigates how military support for the police in Danish domestic security affairs has undergone a recategorization process.
Mette Volquartzen, Rasmus Dahlberg
doaj   +1 more source

Childcare cost and Spanish mother’s labour force participation [PDF]

open access: yes
In Spain, female labour force participation is among the lowest in Europe. This paper analyzes the extent to which female labour force participation is affected by the cost of formal childcare.
Cristina Borra
core  

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

open access: yesFEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold   +11 more
wiley   +1 more source

Humanitarian Necessity: the Problem of Political Legitimation of Uses of Force

open access: yesУправленческое консультирование, 2018
This article explores the problem addressing the political rationale of the «humanitarian intervention» performed in case of emergency and necessity. The practice of states exhibits the emergence of a new international norm that allows states to perform «
Stanislav Valentinovich Korostelev
doaj  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

The Evolution of Views on the Rationale for Uses of Force Prior to the Establishment of Modern Institutions

open access: yesУправленческое консультирование, 2018
This article addresses the issue of justification of uses of force in historical perspective. Formation of the modern doctrine of Just War originated in ancient Greece and Rome.
Stanislav Valentinovich Korostelev
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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