Results 161 to 170 of about 2,258,704 (301)

Chronic pain self-management in middle-aged and older adults: A collective intelligence approach to identifying barriers and user needs in eHealth interventions. [PDF]

Digit Health, 2022
O'Reilly PM, Harney OM, Hogan MJ, Mitchell C, McGuire BE, Slattery B.   +5 more
europepmc   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Understanding User Needs

, 2021
openaire   +1 more source

Understanding users' needs and user centered design

, 2007
This guide (2007) is intended to help organizations, specifically managers and developers, in understanding approaches for user-needs assessments and user-centered designs. User-needs assessments engage user communities to identify the content, technology and policies that will best address issues.
openaire   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Nighttime fears in children: clinical characterisation and the user needs and preferences for a digital parent-led intervention. [PDF]

Child Adolesc Psychiatry Ment Health
Aji M, Datyner A, Gougeon J, Paravastu N, Champ SL, Lim CYS, Teng A, Hudson JL.   +7 more
europepmc   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Putting FUN into involvement: feedback user needs in the design of a mobile phone app for people with long-term conditions. [PDF]

Res Involv Engagem
Clayton DC, Hadjiconstantinou M, Ng AG, Andrade P, Bell J, Eyitemi FD, Hobson R, Masood A, Panagi M, Raza A, Tonga E, Worboys H, P-STEP User Group, Kadam U.   +13 more
europepmc   +1 more source

Pilots for Healthy and Active Ageing (PHArA-ON) Project: Definition of New Technological Solutions for Older People in Italian Pilot Sites Based on Elicited User Needs. [PDF]

Sensors (Basel), 2021
D'Onofrio G, Fiorini L, Toccafondi L, Rovini E, Russo S, Ciccone F, Giuliani F, Sancarlo D, Cavallo F.   +8 more
europepmc   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source