UV written evanescent devices fabricated in micro-structured substrates for optofluidics [PDF]
We report our recent developments in micro-structured evanescent devices. Micromachining prior to direct UV writing of channel waveguides provides additional flexibility to fabricate evanescent interacting devices such as modulators and sensors, which ...
Snow, Benjamin D. +9 more
core +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Potential application and efficiency of UV Light for food preservation
reservedI trattamenti termici sugli alimenti possono danneggiare le caratteristiche organolettiche di questi, per l’azione deleteria delle alte temperature.
COSSARINI, GIULIA
core
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
UV micro-irradiation of the Chinese hamster cell nucleus and caffeine post-treatment immunocytochemical localization of DNA photolesions in cells with partial and generalized chromosome shattering [PDF]
UV micro-irradiation of a small part of the Chinese hamster nucleus and caffeine post-incubation often results in shattered chromosomes at the first post-irradiation mitosis.
Cornelis, Jan J. +8 more
core +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
<p>The UV-CDAT team is pleased to announce the release of UV-CDAT version 2.12</p> <p> </p> <p>UV-CDAT is distributed via anaconda: conda create -n uvcdat-2.12 uvcdat -c conda-forge -c uvcdat Major changes in v2.12 New ...
Thomas Maxwell +12 more
core +1 more source
UV-Curable Polyborosilazane Precursors for SiBCN Coatings under Harsh Environments
UV irradiation is a well-established and effective process for polymer curing. However, it has been largely ignored in the field of polymer-derived ceramics (PDCs) due to its intricate mechanism and strict requirements for the precursor molecular ...
Xiao Zhang (152326) +11 more
core +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source

