Results 191 to 200 of about 308,174 (247)
American Journal of Industrial Medicine, EarlyView.ABSTRACT Background Long COVID affects a significant proportion of COVID‐19 survivors. This study examined persistent Long COVID symptoms among healthcare personnel (HCP) and evaluated associations with vaccination, prior SARS‐CoV‐2 infection, underlying health conditions, and demographics.
Eric Kontowicz +13 more
wiley +1 more source
Site‐Specific Protein Bioconjugation Through Cellular Incorporation of Noncanonical Amino Acids
Angewandte Chemie, EarlyView.Genetic code expansion (GCE) enables site‐specific installation of noncanonical amino acids containing bioorthogonal conjugation handles, allowing precise, homogeneous protein modification. This review examines the principles of orthogonal translation, surveys the chemistries available for chemoselective labeling, and highlights emerging multi‐site ...
Rahul Sarkar +2 more
wiley +2 more sources
American Journal of Medical Genetics Part A, EarlyView.ABSTRACT Primary immunodeficiency disorders may present with gastrointestinal inflammation resembling inflammatory bowel disease (IBD), particularly in patients with pediatric or very‐early‐onset disease. We report a 24‐year‐old male with lifelong immune dysregulation and early‐onset IBD who was ultimately found to carry a hemizygous intronic IL2RG ...
Agustina Fainguersch +9 more
wiley +1 more source
dsRADAR: Imaging and Detecting Cellular dsRNA by Repurposing RNA‐Binding Proteins
Angewandte Chemie, EarlyView.Double‐stranded RNA (dsRNA) plays an important role in the innate immune system, but can be misregulated in disease, leading to inflammation. Despite the importance of this motif, methods for detecting dsRNA remain limited. This work shows that ADAR3 can be repurposed to provide improved detection of dsRNA, which is demonstrated in the context of viral
Weina Cheng +7 more
wiley +2 more sources
Advanced NanoBiomed Research, EarlyView.Engineered endotoxin‐free Escherichia coli were used to assemble poly(3‐hydroxybutyrate) (PHB) biopolymer particles displaying multiple conserved Burkholderia pseudomallei antigens via PhaC‐mediated surface presentation. Following formulation with poly(I:C), the particulate vaccines induced antigen‐specific humoral and cellular immune responses ...
Md. Tanvir Kabir +6 more
wiley +1 more source
Adaptation of a Fluorogenic RNA to N1‐Methylpseudouridine Nucleoside Chemistry
Angewandte Chemie, EarlyView.Several green‐emitting fluorogenic RNA aptamers were evaluated following the replacement of every uridine by N1‐methylpseudouridine (mΨ). Because this modification impaired the function of most aptamers, a directed evolution strategy combining droplet microfluidics, bioinformatics, and rational design was used to identify an aptamer preserving its ...
Patrick P.A. Bertrand +4 more
wiley +2 more sources
Angewandte Chemie, EarlyView.A chemoenzymatic methodology is described that provided a panel of 6‐sulfo sialyl Lewisx‐containing glycans that were probed for receptor specificity of MERS‐CoV. Although the fucoside of 6‐sulfo‐SLex does not directly interact with the spike, it pre‐organizes the molecule into a conformation to allow a salt bridge between the sulfate and the ...
Yunfei Wu +7 more
wiley +2 more sources
Angewandte Chemie International Edition, EarlyView.Organic solvent‐free lipid nanoparticles featuring PEtOx‐based stealth lipids enable efficient mRNA and CRISPR‐Cas9 delivery while exhibiting markedly improved biocompatibility compared with PEG‐based analogues. Their successful ex vivo and in vivo performance, combined with data‐driven formulation optimization, establishes a versatile platform that ...
Michael Streiber +21 more
wiley +1 more source
The tRNA‐Modification Geranyl‐2‐Thiouridine Enables RNA‐Lipid Association
Angewandte Chemie International Edition, EarlyView.We report the preparation of mono‐ to tri‐geranylated RNA and the association of the hydrophobic RNA with membranes for future efficient incorporation of RNA into liposomes. ABSTRACT The packaging of RNA in lipid nanoparticles for protection and delivery was key for the development of mRNA vaccines and therapeutics. Formulation requires the addition of
Johannes Singer +3 more
wiley +1 more source
The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding
Animal Research and One Health, EarlyView.A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang +10 more
wiley +1 more source