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The vacuole

2010
Publisher Summary The vacuole is the most conspicuous organelle in plant cells. It can be defined operationally as a swollen terminally differentiated intracellular membrane-bound compartment of the secretory pathway, and the minimal requirement for the formation of vacuoles is the synthesis of a vacuolar membrane that contains the transporters ...
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Pollen vacuoles and their significance

Planta, 2011
Vacuoles of several types can be observed in pollen throughout its development. Their physiological significance reflects the complexity of the biological process leading to functional pollen grains. Vacuolisation always occurs during pollen development but when ripe pollen is shed the extensive translucent vacuoles present in the vegetative parts in ...
Ettore Pacini   +2 more
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Vacuole biogenesis and protein transport to the plant vacuole: A comparison with the yeast vacuole and the mammalian lysosome

Protoplasma, 1997
The vacuole is often termed the lytic compartment of the plant cell. The yeast cell also possesses a vacuole containing acid hydrolases. In animal cells these enzymes are localized in the lysosome. Recent research suggests that there is good reason to regard these organelles as homologous in terms of protein transport.
David G. Robinson, Giselbert Hinz
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Vacuoles and prevacuolar compartments

Current Opinion in Plant Biology, 2000
Plant vacuoles are complex and dynamic organelles. Important advances have been made in our understanding of the transporters present in the tonoplast and of the molecular interactions that allow targeting to vacuoles. Despite these advances, markers that permit vacuoles to be defined unambiguously have not yet been identified.
Paul C. Bethke, Russell L. Jones
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Studying yeast vacuoles

2002
Publisher Summary This chapter describes methods for studying different aspects of vacuole biogenesis and function. It presents three different approaches for visualizing the yeast vacuole: staining with vital dyes, using green fluorescent protein (GFP)-tagged vacuolar marker proteins, and immunofluorescence microscopy of formaldehyde-fixed cells ...
Tom H. Stevens, Elizabeth Conibear
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The Contractile Vacuole

Nature, 1921
IN connection with previous correspondence on the mode of production of the contractile vacuole in Protozoa (NATURE, vol. cvi., pp. 343, 376, 441), I find that it is, in point of fact, Prof. Marcus Hartog to whom the credit of the osmotic view is to be given. In a communication to the British Association in 1888 (Rep., p. 714) this observer pointed out
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The vacuole in yeast

Proceedings / Indian Academy of Sciences, 1956
1. The vacuole was studied under ordinary, phase contrast and dark ground illumination in yeast of varying ages. With the increasing age of the culture almost all cells appear vacuolated and more and more cells exhibit luminous contours to their vacuoles.
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Isolation of Vacuoles and the Tonoplast

2016
Isolation of various subcellular compartments followed by a high-coverage proteomic analysis provides an unparalleled foundation for the functional analyses of proteins. Analyses of tonoplast preparations free of major contaminants provide insights into vesicular fusion machinery, solute transport, and the vacuole association with the cytoskeleton ...
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Odontoblasts: Vacuoles and Inclusions

Science, 1961
Lipid granules have been found in the cytoplasm of odontoblasts and in the odontoblastic extensions within the dentinal tubules. It is suggested that these granules represent specific activity on the part of the normal cell as well as an increase in number after injury.
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