Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Using Focus Groups to Evaluate a Self-Assessment Approach to Coproduction Value Creation in an International Community of Practice: Phase 2 of the CO-VALUE Study. [PDF]
Forcino RC +3 more
europepmc +1 more source
From Global Value Chains to Local Jobs: Exploring FDI-induced Job Creation in EU-27 [PDF]
Magdalena Olczyk, Marjan Petreski
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Role of inventory and assets in shareholder value creation
Olli‐Pekka Hilmola
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Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Exploring value creation in a virtual community of practice: a framework analysis for knowledge and skills development among primary care professionals. [PDF]
Koatz D +9 more
europepmc +1 more source
Harnessing Technology to Drive Coopetition and Value Co-Creation: A Service-Dominant Perspective [PDF]
Agostinho da Silva +1 more
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Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source

