CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Proposal of a suction approximation to simulate the behaviour of real gases [PDF]
Modelling the gas behaviour is important in reservoir engineering and geomechanical problems related to the analysis of geological energy production and storage, as well as the storage of spent nuclear fuel and high-level radioactive waste.
Tengblad Erik +2 more
doaj +1 more source
Hygrothermal behaviour of timber frame walls finished with a brick veneer cladding [PDF]
In this study, two typical timber frame walls with brick veneer cladding have been constructed at KU Leuven to investigate the hygrothermal response of these constructions in a moderate sea climate.
Langmans, Jelle +4 more
core +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Binding potentials for vapour nanobubbles on surfaces using density functional theory
We calculate density profiles of a simple model fluid in contact with a planar surface using density functional theory (DFT), in particular for the case where there is a vapour layer intruding between the wall and the bulk liquid.
Archer, Andrew J. +2 more
core +1 more source
A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility
ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento +3 more
wiley +1 more source
VAPOUR PRESSURE DEFICIT AND VARIABILITY OF YIELD OF ONION (Allium cepa L.) IN POLAND
Vapour pressure deficit, in addition to light and temperature, is the main meteorological element that influences the degree of transpiration rate, which in turn determines the impact of drought stress on the yield of onions. In this study we attempt to
Robert Kalbarczyk, Eliza Kalbarczyk
doaj
The impact of cigarette/e-cigarette vapour on simulated pulmonary surfactant monolayers under physiologically relevant conditions [PDF]
Deviation in pulmonary surfactant structure–function activity can impair airway patency and lead to respiratory disorders. This novel study aims to evaluate the influence cigarette/e-cigarette vapour has on model surfactant films located within a ...
Akbaş +43 more
core +3 more sources
Pool Evaporation at Higher Vapour Pressures
Hazardous substances with a boiling point close to ambient temperatures will evaporate at higher vapour pressures, so that the evaporation takes places in the smooth transition between the evaporation at boiling point and below boiling point, representing the transition between two different physical phenomena.
Habib, Abdelkarim, Schalau, Bernd
openaire +2 more sources
Thermoreflectance Detection of Point Defects Resulting from Focused Ion Beam Milling
Focused ion beam (FIB) milling is a common tool for nanoscale material processing, however irradiation damage, redeposition, and contamination can occur. We use several characterization tools to show FIB‐induced effects beyond 1 mm from the milled area.
Thomas W. Pfeifer +3 more
wiley +1 more source

