Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Regular and Modified Kernel-Based Estimators of Integrated Variance: The Case with Independent Noise [PDF]
We consider kernel-based estimators of integrated variances in the presence of independent market microstructure effects. We derive the bias and variance properties for all regular kernel-based estimators and derive a lower bound for their asymptotic ...
Neil Shephard +3 more
core
The superiority of likelihood-based confidence interval for variance estimation in a single group. [PDF]
Transl Clin PharmacolJung SM, Kim M, Bae KS.
europepmc +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Invited commentary: mixing multiple imputation and bootstrapping for variance estimation. [PDF]
Am J EpidemiolLi CX, Zivich PN.
europepmc +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Is there a competitive advantage to using multivariate statistical or machine learning methods over the Bross formula in the hdPS framework for bias and variance estimation? [PDF]
PLoS OneEhsanul Karim M, Lei Y.
europepmc +1 more source
Variance estimation when using propensity-score matching with replacement with survival or time-to-event outcomes. [PDF]
Stat Med, 2020 Austin PC, Cafri G.
europepmc +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
SNR and Noise Variance Estimation in Polarimetric SAR Data
, 2013 The problem of estimating the signal-to-noise ratio (SNR) of the cross-polarised channels and the noise variance in polarimetric synthetic aperture radar (SAR) data is dealt with.
Villano, Michelangelo +1 more
core