Results 31 to 40 of about 600,320 (348)

Small RNA sequencing of field Culex mosquitoes identifies patterns of viral infection and the mosquito immune response

open access: yesScientific Reports, 2023
Mosquito-borne disease remains a significant burden on global health. In the United States, the major threat posed by mosquitoes is transmission of arboviruses, including West Nile virus by mosquitoes of the Culex genus.
Steven M. Abel   +9 more
doaj   +1 more source

Efficacy and safety of dihydroartemisinin–piperaquine for the treatment of uncomplicated Plasmodium falciparum and Plasmodium vivax malaria in Papua and Sumatra, Indonesia

open access: yesMalaria Journal, 2022
Background Dihydroartemisinin–piperaquine (DHA–PPQ) has been adopted as first-line therapy for uncomplicated falciparum malaria in Indonesia since 2010.
Puji B. S. Asih   +13 more
doaj   +1 more source

An updated pGREEN-based vector suite for cost-effective cloning in plant molecular biology

open access: yesmicroPublication biology, 2020
Traditional binary shuttle vectors used for creating plant T-DNA insertion mutants can be difficult to work with for several reasons. First, their large size (> 10 kB) and low-copy origin of replication result in low plasmid yields under typical growth conditions. This makes time consuming, and costly, midi or maxi scale plasmid isolation a requirement.
Pratt, Anna I.   +2 more
openaire   +2 more sources

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

open access: yesFrontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
P. Yan   +5 more
semanticscholar   +1 more source

Adenoviral vector‐based platforms for developing effective vaccines to combat respiratory viral infections

open access: yesClinical & Translational Immunology, 2021
Since the development of the first vaccine against smallpox over two centuries ago, vaccination strategies have been at the forefront of significantly impacting the incidences of infectious diseases globally. However, the increase in the human population,
Ahmed Elkashif   +4 more
semanticscholar   +1 more source

Gene amplification by PCR and subcloning into a GFP‐fusion plasmid expression vector as a molecular biology laboratory course* [PDF]

open access: yesBiochemistry and Molecular Biology Education, 2004
AbstractA novel experimental sequence for the advanced undergraduate laboratory course has been developed at Earlham College. Utilizing recent improvements in molecular techniques for a time‐sensitive environment, undergraduates were able to create a chimera of a selected gene and green fluorescent protein (GFP) in a bacterial expression plasmid over ...
Joshua A, Bornhorst   +2 more
openaire   +2 more sources

Thermal biology of invasive Aedes mosquitoes in the context of climate change.

open access: yesCurrent Opinion in Insect Science, 2022
The increasing incidence of arboviral diseases in tropical endemic areas and their emergence in new temperate countries is one of the most important challenges that Public Health agencies are currently facing. Because mosquitoes are poikilotherms, shifts
C. Lahondère, M. Bonizzoni
semanticscholar   +1 more source

Biology Beyond Biochemistry:The Mechanics of Life [PDF]

open access: yes, 2005
In the last half century, biologists have made great strides towards understanding the intricate structure of the cell and the relation between this structure and cellular function.
Wiggins, Paul Alexander
core   +1 more source

Leishmaniasis transmission biology: Role of Promastigote Secretory Gel as a transmission determinant [PDF]

open access: yes
Leishmaniasis is a complex vector-borne disease caused by Leishmania sp. (Kinetoplastida: Trypanosomatidae) and is transmitted by female sand flies (Diptera: Psychodidae).
Martin Sainz de la Maza, O
core   +1 more source

Current Status of Omics Studies Elucidating the Features of Reproductive Biology in Blood-Feeding Insects

open access: yes, 2023
Female insects belonging to the genera Anopheles, Aedes, Glossina, and Rhodnius account for the majority of global vector-borne disease mortality. In response to mating, these female insects undergo several molecular, physiological, and behavioral ...
Aditi Kulkarni   +4 more
core   +1 more source

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