Results 91 to 100 of about 11,997,578 (311)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Experimental study on hydrodynamic characteristics of multiple jets in wave environment

Shui kexue jinzhan, 2016
In order to study the impact of waves on the movement and dilution process of multiple jets,Particle Image Velocimetry(PIV) and Laser Induced Fluorescence(LIF) measurement systems are used to measure velocity field and concentration field under the wave ...
CHEN Yongping, TIAN Wanqing, FANG Jiayu, JIANG Dongbo   +3 more
doaj   +1 more source

A continuous velocity field for Norway

Journal of Geodesy, 2013
In Norway, as in the rest of Fennoscandia, the process of Glacial Isostatic Adjustment causes ongoing crustal deformation. The vertical and horizontal movements of the Earth can be measured to a high degree of precision using GNSS.
H. Kierulf, M. Ouassou, M. Simpson, Olav Vestøl   +3 more
semanticscholar   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Characteristics of plumes in linearly stratified salt-water

Shui kexue jinzhan, 2016
The two-tank setup was employed to produce linearly stratified salt-water in laboratory tank. Laboratory experiments on buoyant plumes were undertaken by jetting dense salt-water downward into the linear stratification,aiming at investigating plume ...
ZHANG Wei, ZHAO Liang, HE Zhiguo, LIN Ting, TAN Liming, JIANG Houshuo   +5 more
doaj   +1 more source

Numerical Simulation of Velocity Field around Two Columns of Tandem Piers of the Longitudinal Bridge

Fluids, 2020
This research explores the effects of different spans of two columns of tandem piers on the characteristics of x-velocity near the river bed based on computational fluid dynamics (CFD) simulations.
Hongliang Qi, Junxing Zheng, Chenguang Zhang   +2 more
doaj   +1 more source

Current plate boundary deformation of the Afar rift from a 3‐D velocity field inversion of InSAR and GPS

, 2014
Extension, faulting, and magmatism are the main controls on the magnitude and localization of strain at mid‐ocean ridges. However, the temporal and spatial patterns of such processes are not clear since the strain distribution has not been resolved in ...
C. Pagli, Hua Wang, T. Wright, É. Calais, E. Lewi   +4 more
semanticscholar   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Recent Crustal Deformation Based on Interpolation of GNSS Velocity in Continental China

Remote Sensing, 2020
We used the interpolation method of two-dimensional vector velocity field data based on Green’s function to conduct coupled interpolation with a Poisson’s ratio of 0.5 for 1966 horizontal velocity field data from 1999 to 2017 and obtained the uniform ...
Weiwei Bian, Jicang Wu, Weiwei Wu
doaj   +1 more source