A Universal Hairy Root Transformation Protocol for Evaluating Editing Efficiency in Dicot Plants
A streamlined workflow for the rapid evaluation of CRISPR‐Cas9 editing efficiency using a universal Agrobacterium rhizogenes–mediated hairy root transformation system. The process encompasses seed germination, explant preparation, bacterial infection, hairy root induction, and comprehensive validation of editing events via Sanger sequencing, PCR ...
Hong Yang +5 more
wiley +1 more source
Macaw Palm Propagation Strategies: Advances, Gaps, and Future Directions for a Promising Oleaginous Crop-A Review. [PDF]
Cavalcanti VP +8 more
europepmc +1 more source
Abstract In this article, we describe an inquiry‐based teaching activity developed in a public high school in Brazil. The objectives were to (a) develop an experimental activity grounded on the students’ autonomy for formulating and testing hypotheses, (b) address Biology topics from a historical and multidisciplinary perspective, (c) stimulate skills ...
Simão Dias Vasconcelos +1 more
wiley +1 more source
Energy-Efficient geopolymer wall panels: optimizing mechanical, thermal, and acoustic properties for sustainable construction. [PDF]
Rathinavel N, Aleem A, Ismail M.
europepmc +1 more source
Evaluation of co-product of vermiculite as substrate in seedlings production of nim
Geraldo Humberto Silva +1 more
openalex +2 more sources
Soil and Genotype Shape the Sugarcane Phytobiome for Enhanced Environmental Adaptation
Soil type and sugarcane genotype, differing in their adaptability to low‐fertility soils, interact to shape microbial recruitment and host transcriptional responses. In sandy soils, the better‐adapted genotype IACSP‐5503 recruits more plant growth‐promoting bacteria and activates growth‐related genes, while in clayey soils the less‐adapted IACSP‐6007 ...
J. D. Ferreti +6 more
wiley +1 more source
A comparative study on acoustical properties using waste recycled porous materials for environmental sustainability. [PDF]
Selvaraj VK +5 more
europepmc +1 more source
Rhizobial motility preference in root colonization of Medicago truncatula
Summary Tunnel‐like infection thread (IT) structures support root colonization by symbiotic nitrogen‐fixing rhizobia bacteria in most legume species. These tip‐grown structures are key to directing rhizobia from root hairs to developing nodules, where they are hosted to fix nitrogen.
Anaïs Delers +11 more
wiley +1 more source
Comprehensive 4E performance assessment of novel porous clay bricks across new climate zones of Türkİye. [PDF]
Acar MŞ +3 more
europepmc +1 more source

