Results 31 to 40 of about 2,110,425 (272)
ScreenworksThis video essay was created as part of the 2024 Parametric Summer Series organized by Ariel Avissar, using "Laird's Constraint" inspired by Colleen Laird's 2023 video essay "Eye-Camera-Ninagawa." Drawing from Laird's work, this piece explores the ...
Quan Zhang
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Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Molecular Oncology, EarlyView.Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez +5 more
wiley +1 more source
ScreenworksThis video essay and accompanying research statement extends and adds to a broader study funded by The Centre for Ageing Better; Screening Older Age: The Representation of Older Adults in British Cinema between 2010 and 2022.
Jonathon Crewe
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Interaction Design and Architecture(s), 2023 The aim of this study was to investigate eighth-grade students’ assessment literacy and writing skills in English as a foreign language using an AI-based automated essay assessment tool (EAT).
Ingeborg Krange +4 more
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Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Film as Sound Art: Embracing Love through Extra-diegetic Sound in Nadine Labaki’s Caramel
Open Screens, 2023 Teaching film often involves un-learning previously taught histories of cinema to find instead ‘herstories’ (Dovey, 2018) and theirstories of cinema. This video essay constitutes an illustrative example of this practice, showing how Lebanese woman-led ...
Estrella Sendra
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SEARCHING SUBJECTIVITIES IN VIDEO INSTALLATIONS OF AMAR KANWAR
Art is on, 2016 The essay views the video installation as an apparatus that lightens multiple subjectivities. By illustrating two works of Amar Kanwar, the essay elaborates on various positions of the audience, the methodologies used by Kanwar in proposing different ...
Charu Maithani
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