Results 51 to 60 of about 4,466,347 (298)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

A Flexible System for Automated Composition of Intelligent Video Analysis [PDF]

open access: yes, 2011
This paper outlines the automatic construction of video processing solutions using multiple software components as opposed to traditional monolithic approaches undertaken by image processing experts.
Spampinato, Concetto   +3 more
core  

New image processing tools for structural dynamic monitoring [PDF]

open access: yes, 2007
This paper presents an introduction to structural damage assessment using image processing on real data (non ideal conditions). Our contribution is much more a groundwork than a classical experimental validation.
J. Morlier   +6 more
core   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

High performance video processing in cloud data centres [PDF]

open access: yes, 2016
Mobile phones and affordable cameras are generating large amounts of video data. This data holds information regarding several activities and incidents. Video analytics systems have been introduced to extract valuable information from this data. However,
Anjum, Ashiq   +3 more
core   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

open access: yesFEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell   +8 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

BeePIV: A Method to Measure Apis Mellifera Traffic with Particle Image Velocimetry in Videos

open access: yesApplied Sciences, 2021
Accurate measurement of honeybee (Apis mellifera) traffic in the vicinity of the hive is critical in systems that continuously monitor honeybee colonies to detect deviations from the norm.
Vladimir Kulyukin   +3 more
doaj   +1 more source

Added Prognostic Value of EEG Reactivity in Comatose Patients Following Cardiac Arrest

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives To evaluate the added prognostic value of EEG reactivity for favorable outcome compared with background analysis during and after targeted temperature management (TTM). Methods Prospective observational cohort study of comatose post–cardiac arrest patients admitted to a single academic center between 2017 and 2022, all undergoing ...
Sarah Caroyer   +11 more
wiley   +1 more source

Fast and Accurate Video Analysis and Visualization of Classroom Activities Using Multiobjective Optimization of Extremely Low-Parameter Models

open access: yesIEEE Access
The paper considers the problem of video activity recognition in real-life collaborative classroom learning environments. Video analysis of real-life collaborative classroom learning environments faces significant challenges not encountered in current ...
Venkatesh Jatla   +4 more
doaj   +1 more source

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