Results 51 to 60 of about 60,942 (313)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

EVALUACIÓN DE LA CALIDAD FISIOLÓGICA DE SEMILLAS COMERCIALES DE Ormosia arborea (Vell.) Harms.

open access: yesHeringeriana, 2015
El objetivo de este trabajo fue evaluar la calidad fisiológica de semillas de Ormosia arborea (Vell.) Harms, fueron utilizadas las pruebas de germinación, tetrazolio, conductividad eléctrica y pH del exudado.
Cibele Carmo da Silva   +5 more
doaj   +3 more sources

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

open access: yesFEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Rapid and simultaneous multiepitope antigen-based detection of Enterococcus by microscale thermophoresis and immunomagnetic separation

open access: yesFrontiers in Microbiology
BackgroundGenerally, enterococci bacteria cause nosocomial infections and are major indicators of bacterial contamination in marine bathing beach. However, a method for the rapid and simultaneous detection of multiple pathogenic enterococci has not been ...
Yan Liu   +19 more
doaj   +1 more source

Vigor e germinação de sementes híbridas de pimenteiras ornamentais

open access: yesOrnamental Horticulture, 2011
Sementes híbridas nem sempre possuem bom vigor e germinação, principalmente quando são resultantes de cruzamentos interespecíficos. Nesse sentido, o objetivo desse trabalho foi avaliar o vigor e a germinação de sementes híbridas de pimenteiras ...
Mayana F. Nascimento   +4 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

open access: yesFEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura   +2 more
wiley   +1 more source

Vigor-S, a new system for evaluating the physiological potential of maize seeds

open access: yesScientia Agricola
: The refinement of vigor tests and the possibility of utilizing computer resources for the effective evaluation of the seed physiological potential have attracted considerable interest from research and seed technologists.
Danielle Otte Carrara Castan   +2 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

open access: yesFEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Efeito dos níveis de vigor e do tratamento fungicida nos testes de germinação e de sanidade de sementes de soja

open access: yesBioscience Journal, 2009
Sementes de soja com diferentes níveis de vigor, tratadas e não tratadas com fungicida foram submetidas ao teste de germinação nos substratos areia e rolo de papel e ao teste de sanidade. No teste de germinação só houve diferenças significativas entre os
Delineide Pereira Gomes   +4 more
doaj  

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