Results 171 to 180 of about 497,332 (389)

Mouse dendritic cells and other myeloid subtypes in healthy lymph nodes and skin: 26‐Color flow cytometry panel for immune phenotyping

open access: yesEuropean Journal of Immunology, Volume 52, Issue 12, Page 2006-2009, December 2022., 2022
This novel 26‐color flow cytometry panel allows the detailed immune phenotyping of the complex network of myeloid cells in murine lymph nodes and skin. With the optimized panel the different murine DC‐subsets and other myeloid cell types can be identified and further characterized for co‐stimulatory and inhibitory surface molecules.
Florian Hornsteiner   +4 more
wiley   +1 more source

pH and conductivity transients during elution of IgG from protein A columns

open access: yesBiotechnology Progress, EarlyView.
Abstract The evolution of pH and conductivity waves during elution of IgG from protein A columns is studied for the resin MabSelect PrismA as well as other commercial resins using glycine and acetate buffers as eluents. The effects of buffer composition, IgG load, and residence time are explored.
Rainer Hahn   +3 more
wiley   +1 more source

Neutrophil activation and neutrophil extracellular traps (NETs) in COVID‐19 ARDS and immunothrombosis

open access: yesEuropean Journal of Immunology, Volume 53, Issue 1, January 2023., 2023
Neutrophils play a role in the immunopathology SARS‐CoV‐2 infection by triggering the formation of NETs, producing cytokines including interleukin‐8 (CXCL8), and mediating the recruitment of other immune cells to regulate inflammation, which can lead to ARDS.
Maria Candida Cesta   +8 more
wiley   +1 more source

Improving outcomes in intensified processing via optimization of the cell line development workflow

open access: yesBiotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi   +8 more
wiley   +1 more source

Pleural CD14+ monocytes/macrophages of healthy adolescents show a high expression of metallothionein family genes

open access: yesEuropean Journal of Immunology, Volume 53, Issue 1, January 2023., 2023
Modern surgical (technical) possibilities enable pleural macrophage isolation and analyses regardless the person`s age. Transcriptome profiling of macrophages from healthy or sick individuals revealed that these cells express high levels of metallothioneins, metal‐binding proteins that are involved in heavy metal detoxification, antioxidative defense ...
Nagoud Schukfeh   +11 more
wiley   +1 more source

The role of packaging sites in efficient and specific virus assembly [PDF]

open access: yesarXiv, 2015
During the lifecycle of many single-stranded RNA viruses, including many human pathogens, a protein shell called the capsid spontaneously assembles around the viral genome. Understanding the mechanisms by which capsid proteins selectively assemble around the viral RNA amidst diverse host RNAs is a key question in virology.
arxiv  

Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments

open access: yesBiotechnology Progress, EarlyView.
Abstract The quality control of RNA has become increasingly crucial with the rise of mRNA‐based vaccines and therapeutics. However, conventional methods such as LC–MS often require specialized equipment and expertise, limiting their applicability to high throughput experiments.
Rochelle Aw, Karen Polizzi
wiley   +1 more source

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