Results 171 to 180 of about 194,914 (262)

Highly Tunable and Cell‐Remodelable Thiol‐ene Alginate‐Peptide Crosslinked Hydrogels to Recreate Cellular and Organoid Microenvironments for Biofabrication

Advanced Healthcare Materials, EarlyView.
Norbornene‐functionalized alginate is crosslinked with a di‐thiolated peptide sequence cleavable by cell‐secreted matrix metalloproteinases and decorated with cell‐adhesion peptides upon exposure to UV. Thyroid follicles, endometrial and intestinal organoids are encapsulated in a gentle manner with high cell viability and correct phenotype ...
Julia Fernández‐Pérez, Adrián Seijas‐Gamardo, Antonio J. Feliciano, Panagiota Kakni, Anna M. Kip, Paul Wieringa, Stefan Giselbrecht, Matthew B. Baker, Lorenzo Moroni   +8 more
wiley   +1 more source

KP177R-based visual assay integrating RPA and CRISPR/Cas12a for the detection of African swine fever virus [PDF]

Haorui Luan, Shujuan Wang, Lin Ju, Tong Liu, Haoyue Shi, Shengqiang Ge, Shijin Jiang, Jiaqiang Wu, Jun Peng   +8 more
openalex   +1 more source

Dual‐Functional Polyphosphoesters for Gene Delivery: Synergistic Effects of Guanidinium and Hydrophobic Side Chains in Degradable Polymers

Advanced Healthcare Materials, EarlyView.
This study presents guanidinium‐ and indole‐functionalized polyphosphoesters as degradable, non‐viral gene delivery vectors. Through precise tuning of charge density and hydrophobicity, these polymers form stable polyplexes with low toxicity. Remarkably, minor structural changes yield up to 200‐fold differences in transfection efficiency, highlighting ...
Markus Kötzsche, Andreas Dzierza, Jan Egger, Timo Rheinberger, Christin Weilandt, Frederik R. Wurm, Ivo Nischang, Dagmar Fischer, Kalina Peneva   +8 more
wiley   +1 more source

POLE3 is a transcriptional repressor of unintegrated linear HIV-1 DNA required for efficient virus integration and escape from innate immune sensing.

, 2023
Benkirane M, Thenin-Houssier S, Jahan C, Machida SM, Bonnet-Madin L, Abbou S, Chen H, Tesfaye R, Cuvier O.   +8 more
europepmc   +1 more source

Analysis of Single-Nucleotide Polymorphisms in Patient-Derived Retrovirus Integration Sites Reveals Contamination from Cell Lines Acutely Infected by Xenotropic Murine Leukemia Virus-Related Virus [PDF]

, 2011
Alice Rusmevichientong, Jaydip Das Gupta, Petra S. Elias, Robert H. Silverman, Samson A. Chow   +4 more
openalex   +1 more source

Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies

Advanced Healthcare Materials, EarlyView.
Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer, Dolores J. Salander, Witold Szymanski, Johannes Graumann, Yukai Wang, Kai Zhao, Jörg W. Bartsch, María Gómez‐Serrano, Daniel Bachurski, Silke Reinartz, Elke von Pogge von Strandmann   +10 more
wiley   +1 more source

Site-Specific Integration in Mammalian Cells Mediated by a New Hybrid Baculovirus–Adeno-Associated Virus Vector [PDF]

, 1998
Fabio Palombo, Andrea Monciotti, Alessandra Recchia, Riccardo Cortese, Gennaro Ciliberto, Nicola La Monica   +5 more
openalex   +1 more source

Kidney Organoids in Drug Development: Integrating Technological Advances and Standardization for Effective Implementation

Advanced Healthcare Materials, EarlyView.
This review examines how emerging enabling technologies enhance the physiological relevance, scalability, and reproducibility of kidney organoids, while advanced analytical approaches support model validation and deepen mechanistic insight into nephrotoxicity.
Helen Kearney, Silvia M. Mihăilă, Lorenzo Moroni, Carlos Mota   +3 more
wiley   +1 more source

4. In Vitro and In Vivo Analysis of Feline Immunodeficiency Virus-Based Lentiviral Vector Integration [PDF]

, 2004

openalex   +1 more source

siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA

Advanced Healthcare Materials, EarlyView.
Osteogenic‐angiogenic cross‐talk is a vital prerequisite for vascularized bone regeneration. In this study, we investigated the effects of siRNA‐mediated silencing of two inhibitory proteins, Chordin and WWP‐1, via CaP‐NP‐loaded gelatin microparticles in osteogenically differentiated microtissues.
Franziska Mitrach, Jonas Kubat, Stefan Simm, Alexandra H. Springwald, Burak Demir, Anton Liebezeit, Michael C. Hacker, Michaela Schulz‐Siegmund   +7 more
wiley   +1 more source